PMID- 8443120
OWN - NLM
STAT- MEDLINE
DCOM- 19930405
LR  - 20190512
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 5
IP  - 1
DP  - 1993 Jan
TI  - Self-restricted primary human histocompatibility leukocyte antigen (HLA)-specific 
      cytotoxic T lymphocytes.
PG  - 103-7
AB  - The specificity of self MHC-restricted T cells responding to peptide fragments of 
      processed antigen has been well characterized. However, the means by which 
      alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We 
      now provide evidence that the recognition of class I human leukocyte antigen 
      (HLA) alloantigen by alloreactive cytotoxic T lymphocytes (CTL) induced in 
      primary mixed lymphocyte cultures is class I HLA-restricted to a great extent. 
      This is shown by the 'split-well' limiting dilution assay using third party 
      target cells bearing a stimulating class I HLA alloantigen either in (i) the 
      presence or (ii) the absence of class I HLA antigen(s) which is shared with the 
      responder. Alloreactive CTL were found which recognize stimulator class I HLA 
      alloantigen on the first but not on the second type of target cells. The results 
      show that, in contrast to the traditional view, a large proportion of the 
      alloreactive CTL against class I HLA alloantigen generated in primary mixed 
      lymphocyte cultures is self class I HLA-restricted.
FAU - Breur-Vriesendorp, B S
AU  - Breur-Vriesendorp BS
AD  - Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, 
      Amsterdam.
FAU - Ivanyi, P
AU  - Ivanyi P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Isoantigens)
SB  - IM
MH  - Cells, Cultured
MH  - Cross Reactions
MH  - HLA-A Antigens/*immunology
MH  - HLA-B Antigens/*immunology
MH  - Humans
MH  - Immunity, Cellular
MH  - Isoantigens/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1093/intimm/5.1.103 [doi]
PST - ppublish
SO  - Int Immunol. 1993 Jan;5(1):103-7. doi: 10.1093/intimm/5.1.103.