PMID- 8445033
OWN - NLM
STAT- MEDLINE
DCOM- 19930405
LR  - 20051117
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 76
IP  - 3
DP  - 1993 Mar
TI  - Tumor necrosis factor-alpha prevents the differentiation of human adipocyte 
      precursor cells and causes delipidation of newly developed fat cells.
PG  - 742-7
AB  - We investigated the effect of the tumor necrosis factor-alpha (TNF alpha) on the 
      differentiation of human adipocyte precursor cells in primary culture. Adipocyte 
      precursors convert into fat cells within 12-16 days in a chemically defined, 
      hormone-supplemented medium. Exposure of cultured preadipocytes to TNF alpha 
      resulted in a dose- and time-dependent decrease in the number of developing fat 
      cells and the activity of glycerol-3-phosphate dehydrogenase (GPDH), an 
      established marker of adipocyte differentiation. A 24-h incubation with TNF alpha 
      at a concentration of 5 nM suppressed GPDH activity by 55% compared to that in 
      control cultures. Continuous exposure of the cells to TNF alpha completely 
      blocked expression of the adipocyte phenotype and GPDH activity. The inhibitory 
      action of TNF alpha was not associated with a change in cell number, as assessed 
      by cell counting. The addition of 5 nM TNF alpha for 24 h to newly developed fat 
      cells caused a rapid reduction of GPDH activity by approximately 50%. A 14-day 
      exposure of differentiated cells to TNF alpha was followed by complete 
      suppression of GPDH and a marked delipidation of the cells, including 
      morphological changes, leading to the development of long spindle-shaped 
      cytoplasmatic extensions. These results clearly demonstrate that TNF alpha 
      inhibits the differentiation of human adipocyte precursor cells and, in addition, 
      promotes the delipidation of mature fat cells. It is suggested that TNF alpha may 
      be involved in the physiological control of human adipose tissue cellularity and 
      function.
FAU - Petruschke, T
AU  - Petruschke T
AD  - Diabetes Research Institute, University of Dusseldorf, Germany.
FAU - Hauner, H
AU  - Hauner H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adipose Tissue/*cytology/drug effects/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - *Lipid Metabolism
MH  - Stem Cells/*cytology/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 10.1210/jcem.76.3.8445033 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1993 Mar;76(3):742-7. doi: 10.1210/jcem.76.3.8445033.