PMID- 8447364
OWN - NLM
STAT- MEDLINE
DCOM- 19930402
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 264
IP  - 2 Pt 1
DP  - 1993 Feb
TI  - Corticosterone induces 11 beta-HSD and mineralocorticoid specificity in an 
      amphibian urinary bladder cell line.
PG  - C317-22
AB  - We have examined the mineralocorticoid specificity in a TBM 18-23 cell line 
      derived from the toad bladder epithelium. In cells grown on porous substrate, 
      corticosterone was more potent than aldosterone in stimulating a sodium transport 
      response, measured by the short-circuit current method 6 h after hormone addition 
      [mean affinity constant (K0.5) for corticosterone = 1 nM vs. K0.5 for aldosterone 
      = 8 nM]. The time course of effects and saturation kinetics were identical for 
      both agonists, suggesting interaction with identical receptors. Whereas the 
      dose-response relationship for aldosterone did not change with time of incubation 
      (6 vs. 24 h), the dose-response curve for corticosterone became biphasic at 24-h 
      incubation (apparent K0.5 as high as 40 nM), demonstrating that corticosterone 
      became apparently less potent with time. Pretreatment with carbenoxolone, a 
      potent inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), restored 
      full sensitivity at 24-h incubation to corticosterone. The 11 beta-HSD activity 
      was low during the first 3 h of incubation in the presence of 3 nM 
      corticosterone, and only a small fraction (approximately 7%) of corticosterone 
      was metabolized. At 24-h incubation, 11 beta-HSD activity increased approximately 
      2.5-fold (P < 0.001, n = 8). We conclude that 11 beta-HSD activity is induced by 
      its own substrate in TBM cells in parallel with the induction of the 
      carbenoxolone-sensitive sodium transport response.
FAU - Gaeggeler, H P
AU  - Gaeggeler HP
AD  - Institut de Pharmacologie et de Toxicologie de l'Universite, Lausanne, 
      Switzerland.
FAU - Duperrex, H
AU  - Duperrex H
FAU - Hautier, S
AU  - Hautier S
FAU - Rossier, B C
AU  - Rossier BC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Mineralocorticoids)
RN  - 4964P6T9RB (Aldosterone)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - MM6384NG73 (Carbenoxolone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Aldosterone/pharmacology
MH  - Animals
MH  - Binding, Competitive
MH  - Biological Transport/drug effects
MH  - Bufo marinus
MH  - Carbenoxolone/metabolism/pharmacology
MH  - Cell Line
MH  - Corticosterone/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Epithelium/metabolism
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Mineralocorticoids/*metabolism
MH  - Sodium/metabolism
MH  - Time Factors
MH  - Urinary Bladder/cytology/*metabolism
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1152/ajpcell.1993.264.2.C317 [doi]
PST - ppublish
SO  - Am J Physiol. 1993 Feb;264(2 Pt 1):C317-22. doi: 10.1152/ajpcell.1993.264.2.C317.