PMID- 8448850
OWN - NLM
STAT- MEDLINE
DCOM- 19930415
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 39
IP  - 3
DP  - 1993 Mar
TI  - Clinical and laboratory studies of a new immunoradiometric assay of parathyroid 
      hormone-related protein.
PG  - 414-9
AB  - Parathyroid hormone-related protein (PTHrP) was measured in plasma by a new 
      immunoradiometric assay (IRMA) from Nichols Institute. The assay is specific for 
      PTHrP and shows excellent parallelism when measuring keratinocyte fluid, samples 
      with high PTHrP content, and PTHrP-supplemented plasma. A precision profile 
      established the assay detection limit at 0.7 pmol/L. PTHrP was unstable in 
      plasma, but the degradation rate was patient-specific. Because delay in 
      separation resulted in loss of PTHrP immunoreactivity, samples were collected 
      into tubes containing protease inhibitors (aprotinin, leupeptin, pepstatin, and 
      EDTA) and separated within 30 min. Among normal subjects, 78% had PTHrP values 
      greater than the detection limit; the reference range established was < 0.7-2.6 
      pmol/L. Of patients with hypercalcemia associated with malignancy, 46% had PTHrP 
      > 2.6 pmol/L. PTHrP was increased in patients with breast (73%), genitourinary 
      (64%), or lung (46%) malignancy but was rarely above normal in patients with 
      hematological (29%) or gastrointestinal (33%) malignancy. PTHrP and nephrogenous 
      cyclic adenosine monophosphate (NcAMP) were strongly correlated (r = 0.63, P < 
      0.01) in 37 patients with PTHrP values greater than the detection limit, but 8 
      patients had PTHrP and parathyroid hormone [PTH(1-84)] values below the limit of 
      detection with inappropriate or increased NcAMP. Five of these eight patients had 
      small cell carcinoma of lung. These patients may have secreted a factor that is 
      not detected by the IRMAs of PTHrP or PTH used in this study but that produces 
      hypercalcemia by means of cAMP-mediated mechanisms.
FAU - Fraser, W D
AU  - Fraser WD
AD  - University Department of Clinical Chemistry, Royal Liverpool University Hospital, 
      UK.
FAU - Robinson, J
AU  - Robinson J
FAU - Lawton, R
AU  - Lawton R
FAU - Durham, B
AU  - Durham B
FAU - Gallacher, S J
AU  - Gallacher SJ
FAU - Boyle, I T
AU  - Boyle IT
FAU - Beastall, G H
AU  - Beastall GH
FAU - Logue, F C
AU  - Logue FC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (PTHLH protein, human)
RN  - 0 (Parathyroid Hormone-Related Protein)
RN  - 0 (Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Humans
MH  - Hyperparathyroidism/blood
MH  - Immunoradiometric Assay/*methods
MH  - Middle Aged
MH  - Neoplasms/blood
MH  - Parathyroid Hormone-Related Protein
MH  - Proteins/*analysis
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1993 Mar;39(3):414-9.