PMID- 8449033
OWN - NLM
STAT- MEDLINE
DCOM- 19930413
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 63
IP  - 1
DP  - 1993
TI  - Fluorescence in situ hybridization combined with immunohistochemistry for highly 
      sensitive detection of chromosome 1 aberrations in neuroblastoma.
PG  - 24-8
AB  - The development and application of a highly sensitive double-target fluorescence 
      in situ hybridization (FISH) method in combination with immunohistochemistry for 
      detection of chromosome 1 abnormalities in interphase nuclei of neuroblastoma 
      samples is reported. An alpha-satellite probe specific for chromosome 1 and a 
      VNTR probe that hybridizes to chromosome band 1p36.3 were hybridized to GD2 
      prestained neuroblastoma cells in double-target FISH experiments. The ratio of 
      intact to deleted chromosome 1 homologs in the neuroblastoma cells was assessed. 
      To demonstrate the reliability of the method described, four selected samples 
      derived from different neuroblastoma stages are presented. FISH results 
      correlated well with data obtained by conventional cytogenetic procedures. The 
      technique described allows sensitive detection of chromosome 1 abnormalities in 
      interphase nuclei and enables partial cytogenetic analysis of nondividing cells 
      with a defined immunological phenotype.
FAU - Strehl, S
AU  - Strehl S
AD  - CCRI, Children's Cancer Research Institute, Vienna, Austria.
FAU - Ambros, P F
AU  - Ambros PF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Antigens, Neoplasm/analysis
MH  - Centromere
MH  - Child, Preschool
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Neuroblastoma/*genetics
MH  - Telomere
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1159/000133494 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1993;63(1):24-8. doi: 10.1159/000133494.