PMID- 8450192
OWN - NLM
STAT- MEDLINE
DCOM- 19930413
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 41
IP  - 4
DP  - 1993 Apr
TI  - Brain-derived neurotrophic factor (BDNF) peptide antibodies: characterization 
      using a Vaccinia virus expression system.
PG  - 521-33
AB  - We describe and characterize a series of polyclonal antibodies, generated against 
      amino acid sequences unique to various regions within pro- and mature 
      brain-derived neurotrophic factor (BDNF), a member of the highly conserved nerve 
      growth factor (NGF) family of neurotrophins. Synthetic peptides were coupled to 
      carrier proteins in the presence of glutaraldehyde to restrict the host animals' 
      immune response to epitopes that are compatible with aldehyde fixation. Initial 
      screenings of the reactivity of the antisera were made on brain sections 
      processed for immunohistochemistry after peptide injections into brain 
      parenchyma. As a means of further characterizing these peptide antisera, we have 
      evaluated the reactivity and specificity of the peptide antibodies in BHK cells 
      expressing recombinant pro- and mature BDNF protein from a T7 RNA 
      polymerase-driven Vaccinia virus system. Several of the antibodies strongly 
      stained components of cells transfected with the BDNF gene but did not label 
      wild-type cells nor cells containing only the expression vector. It has also been 
      possible to detect differential compartmentalization of the BDNF protein at 
      various stages of processing in the BHK cells, as well as in situ in cryostat 
      sections of brain tissue, with antisera to the pro- and mature protein. We 
      conclude that several of our antisera recognize not only the specific peptide 
      immunogens but also what appears to be the corresponding protein native to 
      neurons.
FAU - Wetmore, C J
AU  - Wetmore CJ
AD  - Department of Histology & Neurobiology, Karolinska Institute, Stockholm, Sweden.
FAU - Cao, Y
AU  - Cao Y
FAU - Pettersson, R F
AU  - Pettersson RF
FAU - Olson, L
AU  - Olson L
LA  - eng
GR  - AG04418/AG/NIA NIH HHS/United States
GR  - NS09199/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Immune Sera)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Precursors)
RN  - 0 (Recombinant Proteins)
SB  - IM
GS  - BDNF
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/*immunology
MH  - Antibody Specificity
MH  - Base Sequence
MH  - Brain Chemistry
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Line
MH  - Cricetinae
MH  - Female
MH  - Immune Sera
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/analysis/genetics/*immunology
MH  - Nerve Tissue Proteins/analysis/genetics/*immunology
MH  - Peptide Fragments/immunology
MH  - Protein Precursors/analysis/immunology
MH  - Recombinant Proteins/analysis/immunology
MH  - Transfection
MH  - Vaccinia virus/genetics
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - 10.1177/41.4.8450192 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1993 Apr;41(4):521-33. doi: 10.1177/41.4.8450192.