PMID- 8450766
OWN - NLM
STAT- MEDLINE
DCOM- 19930415
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 8
IP  - 1
DP  - 1993 Jan
TI  - Analysis of micronuclei induced by 2-chlorobenzylidene malonitrile (CS) using 
      fluorescence in situ hybridization with telomeric and centromeric DNA probes, and 
      flow cytometry.
PG  - 35-41
AB  - Micronuclei (MN) induced in NIH 3T3 cells by the tear gas 2-chlorobenzylidene 
      malonitrile (CS) were studied in detail using fluorescence in situ hybridization 
      (FISH). The chromosomal composition of CS-induced MN was analysed by simultaneous 
      use of DNA probes for the telomeric hexamer repeat (TTAGGG) and for mouse major 
      satellite DNA. The majority of CS-induced MN, 63-73% of all CS-induced MN at 
      doses from 10 to 30 microM CS, revealed centromeric signals and several telomeric 
      signals suggesting their origin from whole chromosomes. Almost 50% of all 
      CS-induced MN showed one centromeric signal and were assumed to contain one 
      single chromosome. Only 4.5% of all MN did not show any signal and 23-28% showed 
      telomeric signals only, thus containing acentric fragments. Based on the 
      experimental data from FISH the distribution of the DNA content of CS-induced MN 
      was calculated assuming random breakage of chromosomes, and random combination of 
      chromosomes and chromosome fragments. Good agreement between calculated MN 
      distributions and distributions measured by flow cytometry was obtained. By 
      sorting MN with distinct DNA content and hybridization of the sorted MN with the 
      centromeric probe, regions in the MN distribution containing mainly MN with 
      single whole chromosomes could be demonstrated.
FAU - Miller, B M
AU  - Miller BM
AD  - GSF-Forschungszentrum fur Umwelt- und Gesundheit GmbH, Institut fur 
      Biophysikalische Strahlenforschung, Neuherberg, Germany.
FAU - Nusse, M
AU  - Nusse M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 2698-41-1 (o-Chlorobenzylidenemalonitrile)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Centromere
MH  - Chromosomes/drug effects
MH  - *DNA Probes
MH  - DNA, Satellite
MH  - *Flow Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Micronucleus Tests
MH  - Repetitive Sequences, Nucleic Acid
MH  - Telomere
MH  - o-Chlorobenzylidenemalonitrile/*pharmacology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1093/mutage/8.1.35 [doi]
PST - ppublish
SO  - Mutagenesis. 1993 Jan;8(1):35-41. doi: 10.1093/mutage/8.1.35.