PMID- 8454364
OWN - NLM
STAT- MEDLINE
DCOM- 19930422
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 61
IP  - 4
DP  - 1993 Apr
TI  - Cloning and sequence analysis of the structural pilin gene of Brazilian purpuric 
      fever-associated Haemophilus influenzae biogroup aegyptius.
PG  - 1559-62
AB  - We have cloned and sequenced the Brazilian purpuric fever (BPF)-associated 
      Haemophilus influenzae biogroup aegyptius (Hae) pilin gene. The sequence 
      contained a 648-bp open reading frame encoding a mature pilin protein of 191 
      amino acids with a calculated mass of 20.5 kDa. There was 82% homology between 
      the open reading frames of the BPF strain F3031 and H. influenzae type b (Hib) 
      (strain M43) pilin genes and 71% homology at the amino acid level between the 
      mature pilin proteins. However, areas of diversity were noted throughout the 
      gene. A 17-bp probe corresponding to an area of diversity in the N-terminal 
      region of the BPF-associated gene hybridized with other BPF strains but not with 
      non-BPF Hae or Hib. In summary, the pilin protein of BPF-associated Hae is highly 
      homologous to Hib pilin yet remains structurally distinct.
FAU - Whitney, A M
AU  - Whitney AM
AD  - Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 
      30303.
FAU - Farley, M M
AU  - Farley MM
LA  - eng
SI  - GENBANK/L08606
GR  - 507 RR05364/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Proteins)
RN  - 147680-16-8 (Fimbriae Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Outer Membrane Proteins/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Fimbriae Proteins
MH  - Genes, Bacterial
MH  - Haemophilus Infections/*microbiology
MH  - Haemophilus influenzae/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry
MH  - Purpura
MH  - Recombinant Proteins
MH  - Sequence Alignment
PMC - PMC281401
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
PMCR- 1993/04/01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PHST- 1993/04/01 00:00 [pmc-release]
AID - 10.1128/iai.61.4.1559-1562.1993 [doi]
PST - ppublish
SO  - Infect Immun. 1993 Apr;61(4):1559-62. doi: 10.1128/iai.61.4.1559-1562.1993.