PMID- 8455135
OWN - NLM
STAT- MEDLINE
DCOM- 19930421
LR  - 20170214
IS  - 0885-3282 (Print)
IS  - 0885-3282 (Linking)
VI  - 7
IP  - 3
DP  - 1993 Jan
TI  - Relationship of surface characteristics to cellular attachment in PU and PHEMA.
PG  - 250-64
AB  - Adhesion characteristics of fibroblastic baby hamster kidney (BHK) cells and 
      epithelial Madine Darby kidney (MDBK) cells on polyurethane (PU) and 
      polyhydroxyethylmethacrylate (PHEMA) based surfaces have been studied. PU 
      surfaces were prepared by a classical solvent-casting procedure of Pellethane 
      solution that contains different types of solvents, i.e., THF, dioxane and their 
      compositions. PHEMA based surfaces were obtained by bulk polymerization of 
      respective comonomers (HEMA, acrylic acid, AA, and 
      dimethylaminoethylmethacrylate, DMAEMA) in the presence of the crosslinker. Thus, 
      a number of polymeric surfaces were obtained with different surface charges (COO- 
      and NH+4) and with different surface free energies in a range between 60-82 
      ergs/cm2. Surface properties of these membranes were characterized by equilibrium 
      water contents, air and octane contact angles, surface free energies, SEM 
      photographs and ATR-FTIR spectra. Interactions of BHK and MDBK cells with the 
      surfaces were examined in stationary culture conditions which were carried out in 
      MEM supplemented with fetal calf serum. The observations strongly suggested that 
      the chemical and/or physical properties of membrane surface and morphology of the 
      cell control the degree of cell adhesion to the PU and PHEMA based membranes.
FAU - Kiremitci, M
AU  - Kiremitci M
AD  - Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
FAU - Pesmen, A
AU  - Pesmen A
FAU - Pulat, M
AU  - Pulat M
FAU - Gurhan, I
AU  - Gurhan I
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polyurethanes)
RN  - 25249-16-5 (Polyhydroxyethyl Methacrylate)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/*drug effects
MH  - Cell Line
MH  - Cells, Cultured/drug effects/ultrastructure
MH  - Cricetinae
MH  - Epithelium/drug effects/ultrastructure
MH  - Fibroblasts/drug effects/ultrastructure
MH  - Fourier Analysis
MH  - Kidney/cytology
MH  - Membranes, Artificial
MH  - Microscopy, Electron, Scanning
MH  - Polyhydroxyethyl Methacrylate/*pharmacology
MH  - Polyurethanes/*pharmacology
MH  - Spectrophotometry, Infrared/methods
MH  - Surface Properties
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1177/088532829300700304 [doi]
PST - ppublish
SO  - J Biomater Appl. 1993 Jan;7(3):250-64. doi: 10.1177/088532829300700304.