PMID- 8458413
OWN - NLM
STAT- MEDLINE
DCOM- 19930428
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 319
IP  - 3
DP  - 1993 Mar 22
TI  - Inhibition of passive sensitization of human peripheral basophils by synthetic 
      human immunoglobulin E peptide fragments.
PG  - 225-8
AB  - To delineate the binding site in the human immunoglobulin E (IgE) molecule to the 
      Fc epsilon receptor on basophils and mast cells, we chemically synthesized a 
      total of 71 peptide fragments within the sequence Ser300-Lys547 in the human IgE 
      molecule. The synthetic peptides were tested for their capacity to inhibit 
      passive sensitization of human peripheral basophils with atopic patient's serum 
      containing the specific IgE against dust mites in vitro. It was found that a 
      peptide fragment, Pro345-Ile356, potently inhibited the passive sensitization. To 
      clarify the minimal active core, various analogues, such as shortened, 
      substituted (by Gly or Ala residue), omission and retro-sequence peptides, were 
      synthesized and assayed. The results suggested that the sequence Pro345-Lys352 in 
      the human IgE molecule would be an IgE binding site, and that a synthetic 
      octapeptide, Pro345-Phe-Asp-Leu-Phe-Ile-Arg-Lys352, inhibited the passive 
      sensitization, probably by occupying the Fc epsilon receptor sites on the cells.
FAU - Nio, N
AU  - Nio N
AD  - Central Research Laboratories, Ajinomoto Co., Kawasaki, Japan.
FAU - Seguro, K
AU  - Seguro K
FAU - Ariyoshi, Y
AU  - Ariyoshi Y
FAU - Imano, K
AU  - Imano K
FAU - Yakuo, I
AU  - Yakuo I
FAU - Kita, A
AU  - Kita A
FAU - Nakamura, H
AU  - Nakamura H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Allergens)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Amino Acid Sequence
MH  - Animals
MH  - Basophils/*immunology
MH  - Humans
MH  - Hypersensitivity
MH  - Immunization, Passive
MH  - Immunoglobulin E/chemistry/*immunology
MH  - In Vitro Techniques
MH  - Mites/immunology
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/immunology
MH  - Receptors, IgE/*metabolism
MH  - Sequence Alignment
EDAT- 1993/03/22 00:00
MHDA- 1993/03/22 00:01
CRDT- 1993/03/22 00:00
PHST- 1993/03/22 00:00 [pubmed]
PHST- 1993/03/22 00:01 [medline]
PHST- 1993/03/22 00:00 [entrez]
AID - 0014-5793(93)80551-5 [pii]
AID - 10.1016/0014-5793(93)80551-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1993 Mar 22;319(3):225-8. doi: 10.1016/0014-5793(93)80551-5.