PMID- 8461137
OWN - NLM
STAT- MEDLINE
DCOM- 19930504
LR  - 20220309
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 10
IP  - 3
DP  - 1993 Mar
TI  - Multiple promoters direct tissue-specific expression of the rat BDNF gene.
PG  - 475-89
AB  - Brain-derived neurotrophic factor (BDNF) supports the survival of a specific set 
      of neurons in the vertebrate nervous system. Here we show that the rat BDNF gene 
      consists of four short 5' exons and one 3' exon encoding the mature BDNF protein. 
      Eight different BDNF mRNAs with four different 5' ends and two alternative 
      polyadenylation sites are transcribed from this gene. BDNF mRNAs containing exons 
      I, II, and III are expressed predominantly in the brain, whereas exon IV 
      transcripts predominate in the lung and heart. mRNAs containing exons I, II, and 
      III increase markedly in the brain after kainic acid-induced seizures, whereas 
      exon IV mRNA increases only slightly. Several transcription initiation sites were 
      mapped upstream of the four 5' exons, and transfection of promoter-reporter gene 
      constructs confirmed that these sequences act as promoters. Combined, the data 
      demonstrate that alternative usage of four promoters within the BDNF gene and 
      differential splicing control tissue-specific and seizure-induced expression of 
      BDNF mRNA.
FAU - Timmusk, T
AU  - Timmusk T
AD  - Department of Medical Chemistry (II), Karolinska Institute, Stockholm, Sweden.
FAU - Palm, K
AU  - Palm K
FAU - Metsis, M
AU  - Metsis M
FAU - Reintam, T
AU  - Reintam T
FAU - Paalme, V
AU  - Paalme V
FAU - Saarma, M
AU  - Saarma M
FAU - Persson, H
AU  - Persson H
LA  - eng
SI  - GENBANK/X67106
SI  - GENBANK/X67107
SI  - GENBANK/X67108
GR  - AG 04418/AG/NIA NIH HHS/United States
GR  - NS 09199/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Line, Transformed
MH  - DNA/genetics
MH  - Exons
MH  - *Gene Expression
MH  - In Situ Hybridization
MH  - Kainic Acid/pharmacology
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Oligonucleotide Probes/genetics
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 0896-6273(93)90335-O [pii]
AID - 10.1016/0896-6273(93)90335-o [doi]
PST - ppublish
SO  - Neuron. 1993 Mar;10(3):475-89. doi: 10.1016/0896-6273(93)90335-o.