PMID- 8463242
OWN - NLM
STAT- MEDLINE
DCOM- 19930505
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 268
IP  - 10
DP  - 1993 Apr 5
TI  - Characterization of the mouse carbonic anhydrase II gene promoter.
PG  - 7081-9
AB  - This work characterizes a 250-base pair (bp) fragment of the mouse carbonic 
      anhydrase II (CAII) gene as an efficient but not cell-specific promoter. This 
      fragment contains multiple consensus cis regulatory regions that interact in a 
      complex fashion to regulate transcriptional activity from the promoter. Truncated 
      fragments of the 250-bp promoter retain transcriptional activity. The 90-bp 5' 
      "GC"-rich portion of the promoter can direct transcription independently from the 
      consensus TATA box and also contains a silencer that diminishes transcription 
      from the 3' 160-bp portion of the CAII promoter as well as from the SV40 
      promoter. There are two nonconsensus dual functioning Ap2-like elements on the 
      promoter that are essential for core promoter activity and cAMP-mediated 
      increases in transcription of the gene. A nuclear protein of approximately 65 kDa 
      binds to these elements and is present in nuclear extracts of nonstimulated and 
      forskolin-stimulated NIH-3T3 cells. I conclude that these nonconsensus Ap2-like 
      elements and their cognitive binding protein play a major role in the expression 
      of the CAII gene.
FAU - Marino, L R
AU  - Marino LR
AD  - University of Michigan Medical Center, University of Michigan Peptide Hormone 
      Center, Ann Arbor 48109.
LA  - eng
GR  - 2P30 DK34933/DK/NIDDK NIH HHS/United States
GR  - H08 HD00760/HD/NICHD NIH HHS/United States
GR  - R01 HD25983/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 1F7A44V6OU (Colforsin)
RN  - 9007-49-2 (DNA)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 3.1.21.4 (CTGCAG-specific type II deoxyribonucleases)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
GS  - CAII
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Carbonic Anhydrases/*genetics/metabolism
MH  - Chloramphenicol O-Acetyltransferase/genetics/metabolism
MH  - Cloning, Molecular
MH  - Colforsin/pharmacology
MH  - DNA
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - *Promoter Regions, Genetic
MH  - Restriction Mapping
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1993/04/05 00:00
MHDA- 1993/04/05 00:01
CRDT- 1993/04/05 00:00
PHST- 1993/04/05 00:00 [pubmed]
PHST- 1993/04/05 00:01 [medline]
PHST- 1993/04/05 00:00 [entrez]
AID - S0021-9258(18)53148-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1993 Apr 5;268(10):7081-9.