PMID- 8464232
OWN - NLM
STAT- MEDLINE
DCOM- 19930506
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 7
IP  - 4
DP  - 1993 Apr
TI  - Interphase in situ hybridization reveals minimal residual disease in early 
      remission and return of the diagnostic clone in karyotypically normal relapse of 
      acute lymphoblastic leukemia.
PG  - 537-43
AB  - Early clinical remission of acute lymphoblastic leukemia (ALL) was examined by 
      fluorescence in situ hybridization (FISH) in bone marrow cells from eight 
      patients with high hyperdiploid (> 50 chromosomes) clones at diagnosis. Alphoid 
      centromeric probes to chromosomes X, 10, 17, and 18, trisomic at diagnosis, were 
      used as appropriate. Three hematologically normal marrows were used as controls. 
      At diagnosis, trisomic interphase cells ranged from 69.3-84.4%. One month later, 
      trisomic and tetrasomic interphase cells were significantly increased over 
      control frequencies in 2/7 cases tested (p < 0.05 and p < 0.001) and trisomy 
      alone in one case (p < 0.05). At two months post-diagnosis, trisomy and tetrasomy 
      were in the control range. Pentasomy and hexasomy, not seen in controls, were 
      found in 5/7 samples at one month and in 1/5 samples at two months. One patient 
      examined in chromosomally normal relapse had 34.4% trisomic cells by FISH at 
      confirmed relapse (p < 0.001). An additional hyperdiploid case, examined at 
      central nervous system relapse, had increased numbers of trisomic cells (p < 
      0.01) in a morphologically and cytogenetically normal marrow. These findings 
      demonstrate the elimination of aberrant ploidy in most hyperdiploid cases two 
      months from diagnosis and indicate that failure to detect the abnormal clone in 
      relapse may result from selective mitotic activity of chromosomally normal cells, 
      rather than relapse in a cytogenetically normal clone.
FAU - Heerema, N A
AU  - Heerema NA
AD  - Department of Haematology, Royal Free Hospital and School of Medicine, London, 
      UK.
FAU - Argyropoulos, G
AU  - Argyropoulos G
FAU - Weetman, R
AU  - Weetman R
FAU - Tricot, G
AU  - Tricot G
FAU - Secker-Walker, L M
AU  - Secker-Walker LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Bone Marrow/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis
MH  - Recurrence
MH  - Remission Induction
MH  - Trisomy
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1993 Apr;7(4):537-43.