PMID- 8464867
OWN - NLM
STAT- MEDLINE
DCOM- 19930504
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 7
DP  - 1993 Apr 1
TI  - Neurotrophin 3 supports the survival of developing muscle sensory neurons in 
      culture.
PG  - 2613-7
AB  - Target-dependent cell death of different sub-populations of sensory neurons may 
      be regulated by different trophic factors. To investigate this possibility, we 
      have taken advantage of the fact that the fractions of muscle sensory and 
      cutaneous sensory neurons in chicken dorsal root ganglia (DRG) are probably 
      different at different segmental levels, and we have compared the responses of 
      chicken DRG from levels that do and do not innervate limb tissue to various 
      growth factors in vitro. Nerve growth factor (NGF) and brain-derived neurotrophic 
      factor (BDNF) both supported neurite outgrowth from DRG explanted from all 
      segmental levels. In contrast, neurotrophin 3 (NT-3) supported robust neurite 
      growth only from DRG explanted from the cervical or lumbar levels, which 
      innervate limb muscles. Similarly, NGF and BDNF both promoted survival of 
      dissociated neurons from limb and nonlimb segmental levels, whereas NT-3 promoted 
      survival of more neurons from limb compared to nonlimb levels. This suggests that 
      muscle sensory neurons, which are probably more prevalent at the cervical and 
      lumbar levels, may be specifically affected by NT-3. To evaluate this possibility 
      directly, we compared the survival of retrogradely labeled muscle and cutaneous 
      neurons in NGF, BDNF, and NT-3. Identified muscle sensory neurons survived best 
      in vitro in the presence of NT-3, while the survival of identified cutaneous 
      sensory neurons was greatest in NGF. This work provides direct evidence for a 
      potential role of NT-3 versus NGF in the survival of a specific subpopulation of 
      DRG neurons.
FAU - Hory-Lee, F
AU  - Hory-Lee F
AD  - Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh 
      School of Medicine, PA 15213.
FAU - Russell, M
AU  - Russell M
FAU - Lindsay, R M
AU  - Lindsay RM
FAU - Frank, E
AU  - Frank E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Tissue Extracts)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Dose-Response Relationship, Drug
MH  - Ganglia, Spinal/*cytology
MH  - Muscles/*innervation/physiology
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neurites/drug effects/ultrastructure
MH  - Neurons, Afferent/*cytology/drug effects
MH  - Neurotrophin 3
MH  - Skin/innervation
MH  - Spinal Cord/*cytology
MH  - Tissue Extracts/pharmacology
PMC - PMC46145
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
PMCR- 1993/10/01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PHST- 1993/10/01 00:00 [pmc-release]
AID - 10.1073/pnas.90.7.2613 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2613-7. doi: 10.1073/pnas.90.7.2613.