PMID- 8467794
OWN - NLM
STAT- MEDLINE
DCOM- 19930513
LR  - 20231213
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 12
IP  - 4
DP  - 1993 Apr
TI  - Cloned transcription factor MTF-1 activates the mouse metallothionein I promoter.
PG  - 1355-62
AB  - Metallothioneins (MTs) are small cysteine-rich proteins whose structure is 
      conserved from fungi to man. MTs strongly bind heavy metals, notably zinc, copper 
      and cadmium. Upon exposure of cells to heavy metal and other adverse treatments, 
      MT gene transcription is strongly enhanced. Metal induction is mediated by 
      several copies of a 15 bp consensus sequence (metal-responsive element, MRE) 
      present in the promoter region of MT genes. We and others have demonstrated the 
      presence of an MRE-binding factor in HeLa cell nuclear extracts. We found that 
      this factor, termed MTF-1 (MRE-binding transcription factor) is 
      inactivated/reactivated in vitro by zinc withdrawal/addition. Here we report that 
      the amounts of MTF-1-DNA complexes are elevated several-fold in zinc-treated 
      cells, as measured by bandshift assay. We have also cloned the cDNA of mouse 
      MTF-1, a 72.5 kDa protein. MTF-1 contains six zinc fingers and separate 
      transcriptional activation domains with high contents of acidic and proline 
      residues. Ectopic expression of MTF-1 in primate or rodent cells strongly 
      enhances transcription of a reporter gene that is driven by four consensus MREd 
      sites, or by the complete mouse MT-I promoter, even at normal zinc levels.
FAU - Radtke, F
AU  - Radtke F
AD  - Universitat Zurich, Institut fur Molekularbiologie II, Switzerland.
FAU - Heuchel, R
AU  - Heuchel R
FAU - Georgiev, O
AU  - Georgiev O
FAU - Hergersberg, M
AU  - Hergersberg M
FAU - Gariglio, M
AU  - Gariglio M
FAU - Dembic, Z
AU  - Dembic Z
FAU - Schaffner, W
AU  - Schaffner W
LA  - eng
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 9038-94-2 (Metallothionein)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics
MH  - Gene Expression
MH  - Metallothionein/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry/metabolism
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins/metabolism
MH  - Transcription Factors/*genetics
MH  - Zinc/physiology
MH  - Zinc Fingers
MH  - Transcription Factor MTF-1
PMC - PMC413347
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
PMCR- 1994/04/01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PHST- 1994/04/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1993.tb05780.x [doi]
PST - ppublish
SO  - EMBO J. 1993 Apr;12(4):1355-62. doi: 10.1002/j.1460-2075.1993.tb05780.x.