PMID- 8469387
OWN - NLM
STAT- MEDLINE
DCOM- 19930513
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 149
IP  - 1
DP  - 1993 Jan 4
TI  - Extension of optic nerve fibers on genetically modified cells producing 
      brain-derived neurotrophic factor.
PG  - 83-6
AB  - Since brain-derived neurotrophic factor (BDNF) promotes the survival of retinal 
      ganglion cells (RGCs), we transfected a rat BDNF cDNA into rat fibroblasts, and 
      retinal fragments of rat embryos were cultured on cell monolayers of these cells. 
      Retinal fragments showed enhanced neurite extension on BDNF-transfected cells 
      compared with that on control cells. The degree of the neurite extension, 
      however, decreased depending upon the embryonic stages. These results suggest 
      that fibroblasts genetically modified to produce BDNF might be a promoter of 
      neurite extension by RGCs, but this does not apply to the RGCs of late embryonic 
      stages.
FAU - Takahashi, J B
AU  - Takahashi JB
AD  - Department of Viral Oncology, Kyoto University, Japan.
FAU - Hoshimaru, M
AU  - Hoshimaru M
FAU - Kikuchi, H
AU  - Kikuchi H
FAU - Hatanaka, M
AU  - Hatanaka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Line, Transformed
MH  - DNA
MH  - Embryo, Mammalian/physiology
MH  - Embryo, Nonmammalian
MH  - Fibroblasts/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Histological Techniques
MH  - Nerve Fibers/physiology
MH  - Nerve Growth Factors/metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurites/physiology
MH  - Retina/embryology/*physiology
MH  - *Transfection
EDAT- 1993/01/04 00:00
MHDA- 1993/01/04 00:01
CRDT- 1993/01/04 00:00
PHST- 1993/01/04 00:00 [pubmed]
PHST- 1993/01/04 00:01 [medline]
PHST- 1993/01/04 00:00 [entrez]
AID - 0304-3940(93)90353-M [pii]
AID - 10.1016/0304-3940(93)90353-m [doi]
PST - ppublish
SO  - Neurosci Lett. 1993 Jan 4;149(1):83-6. doi: 10.1016/0304-3940(93)90353-m.