PMID- 8471253
OWN - NLM
STAT- MEDLINE
DCOM- 19930518
LR  - 20041117
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 34
IP  - 3
DP  - 1993 Mar
TI  - Simultaneous detection of X- and Y-bearing human sperm by double fluorescence in 
      situ hybridization.
PG  - 308-13
AB  - Double fluorescence in situ hybridization (FISH) was used to detect sex 
      chromosomes in decondensed human sperm nuclei. Biotinylated X chromosome specific 
      (TRX) and digoxigenin-labeled Y chromosome specific (HRY) probes were 
      simultaneously hybridized to sperm preparations from 12 normal healthy donors. 
      After the hybridization, the probes were detected immunocytochemically, using two 
      different and independent affinity systems. Ninety-six percent of the 12,636 
      sperm showed fluorescent labeling, of which 47.4% were haploid X and 46.8% were 
      haploid Y. A frequency of 0.46% of XX-bearing sperm (0.28% disomic, 0.18% 
      diploid) and 0.38% YY-bearing sperm (0.21% disomic, 0.17% diploid) was found. The 
      overall proportions of X- and Y-bearing sperm in the ejaculates were 47.9% and 
      47.2%, respectively, which was not significantly different from the expected 
      50:50 ratio. In addition 0.21% of cells appeared to be haploid XY-bearing sperm, 
      0.62% were diploid XY-bearing cells, and 0.05% of cells were considered to be 
      tetraploid cells. The application of double FISH to human sperm using 
      X-chromosome and Y-chromosome probes has allowed a more accurate assessment of 
      the sex chromosal complements in sperm than single FISH method and quinacrine 
      staining for Y-bodies.
FAU - Han, T L
AU  - Han TL
AD  - Genetics Department, University of Adelaide, Queen Elizabeth Hospital, Woodville, 
      South Australia.
FAU - Ford, J H
AU  - Ford JH
FAU - Webb, G C
AU  - Webb GC
FAU - Flaherty, S P
AU  - Flaherty SP
FAU - Correll, A
AU  - Correll A
FAU - Matthews, C D
AU  - Matthews CD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - DNA Probes
MH  - Diploidy
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Polyploidy
MH  - Spermatozoa/*ultrastructure
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 10.1002/mrd.1080340311 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1993 Mar;34(3):308-13. doi: 10.1002/mrd.1080340311.