PMID- 8471846
OWN - NLM
STAT- MEDLINE
DCOM- 19930520
LR  - 20060421
IS  - 1046-5928 (Print)
IS  - 1046-5928 (Linking)
VI  - 4
IP  - 2
DP  - 1993 Apr
TI  - Recombinant human transforming growth factor-beta 1: expression by Chinese 
      hamster ovary cells, isolation, and characterization.
PG  - 130-40
AB  - A partial cDNA clone encoding the gene for human transforming growth factor-beta 
      1 (TGF-beta 1) was isolated from a human bladder carcinoma cell line (5637) cDNA 
      library. Following restriction enzyme processing and ligation of synthetic 
      oligonucleotide linkers, the gene was inserted into a plasmid and transfected 
      into Chinese hamster ovary cells. Clonal selection and growth conditions resulted 
      in a method for production of recombinant human TGF-beta 1 at 7 mg/liter in 
      conditioned cell medium. Through a combination of low pH treatment, 
      cation-exchange chromatography, and salt precipitation, the recombinant human 
      TGF-beta 1 was purified in milligram amounts to > 95% purity in a yield of about 
      36%. Purification to homogeneity was accomplished by chromatography on C18 silica 
      gel. Amino acid analysis, N-terminal sequencing, and growth inhibition assays 
      indicate identity with the molecule from human platelets.
FAU - Bourdrel, L
AU  - Bourdrel L
AD  - Amgen, Inc., Amgen Center, Thousand Oaks, California 91320.
FAU - Lin, C H
AU  - Lin CH
FAU - Lauren, S L
AU  - Lauren SL
FAU - Elmore, R H
AU  - Elmore RH
FAU - Sugarman, B J
AU  - Sugarman BJ
FAU - Hu, S
AU  - Hu S
FAU - Westcott, K R
AU  - Westcott KR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Animals
MH  - Base Sequence
MH  - Biological Assay/methods
MH  - CHO Cells
MH  - Cell Division/drug effects
MH  - Cell Line, Transformed
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Ion Exchange
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Culture Media
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Sequence Analysis
MH  - Transfection
MH  - Transforming Growth Factor beta/biosynthesis/*genetics/isolation & purification
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - S1046-5928(83)71019-3 [pii]
AID - 10.1006/prep.1993.1019 [doi]
PST - ppublish
SO  - Protein Expr Purif. 1993 Apr;4(2):130-40. doi: 10.1006/prep.1993.1019.