PMID- 8473417
OWN - NLM
STAT- MEDLINE
DCOM- 19930519
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 8
IP  - 2
DP  - 1993 Feb
TI  - Induction of a polarized micro-environment by human T cells and interferon-gamma 
      in three-dimensional spheroid cultures of human endometrial epithelial cells.
PG  - 182-92
AB  - Expression of human leukocyte antigen (HLA)-DR molecules and proliferation of 
      epithelium in human endometrium are polarized. We have suggested that the 
      induction of such a polarized micro-environment is T cell and interferon 
      (IFN)-gamma dependent. The present study was designed to demonstrate the 
      induction of such a micro-environment around T cells and around the source of 
      IFN-gamma. Spheroids reminiscent of endometrial glands were formed by allowing 
      three-dimensional aggregation of endometrial epithelial cells of a cloned HLA-DR 
      negative endometrial carcinoma cell line (ECC1) over agarose. Both HLA-DR 
      expression and inhibition of proliferation were found to be directly dependent on 
      the dose of IFN-gamma that was allowed to diffuse in the agarose beneath the 
      spheroids. To show that the interaction of the epithelial cells with activated T 
      cells also induces HLA-DR molecules in a paracrine fashion in the epithelial 
      cells, ECC1 spheroids were co-cultured with increasing numbers of allogeneic 
      peripheral blood T cells for various time-intervals. T cells bound to the ECC1 
      cells, and became activated as indicated by the expression of interleukin (IL)-2 
      receptor and HLA-DR molecules. A focal HLA-DR expression became apparent in the 
      ECC1 cells adjacent to the T cells. As the number of T cells added to spheroid 
      cultures was increased, a concomitant increase in the number of HLA-DR positive 
      ECC1 cells occurred and HLA-DR immunoreactivity was enhanced in each cell. There 
      was a corresponding decrease in the proliferation of the ECC1 cells in T 
      cell-ECC1 spheroid co-cultures. Based on these data, we suggest that activation 
      of T cells is associated with the induction of HLA-DR expression and inhibition 
      of proliferation in a paracrine fashion in the epithelial cells and may be 
      responsible for the creation of a polarized micro-environment in vivo.
FAU - Tabibzadeh, S
AU  - Tabibzadeh S
AD  - Department of Pathology, University of South Florida, Tampa 33612.
FAU - Sun, X Z
AU  - Sun XZ
FAU - Kong, Q F
AU  - Kong QF
FAU - Kasnic, G
AU  - Kasnic G
FAU - Miller, J
AU  - Miller J
FAU - Satyaswaroop, P G
AU  - Satyaswaroop PG
LA  - eng
GR  - CA46349/CA/NCI NIH HHS/United States
GR  - CA46866/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (HLA-DR Antigens)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects/immunology
MH  - Cell Line
MH  - Cell Polarity/drug effects/*immunology
MH  - Endometrium/cytology/drug effects/*immunology
MH  - Epithelial Cells
MH  - Epithelium/drug effects/immunology
MH  - Female
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Mice
MH  - Sepharose
MH  - T-Lymphocytes/*immunology
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a138020 [doi]
PST - ppublish
SO  - Hum Reprod. 1993 Feb;8(2):182-92. doi: 10.1093/oxfordjournals.humrep.a138020.