PMID- 8473750
OWN - NLM
STAT- MEDLINE
DCOM- 19930520
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 150
IP  - 9
DP  - 1993 May 1
TI  - Heterologous desensitization of the high affinity receptor for IgE (Fc epsilon 
      R1) on RBL cells.
PG  - 4072-83
AB  - To study the properties of Fc epsilon RI desensitization induced by aggregation 
      of that receptor, RBL cells were sensitized with a mixture of two different IgE 
      mAb to create two different populations of IgE-receptor complexes. Cross-linking 
      of one receptor population containing anti-dinitrophenyl (DNP) IgE with a 
      bivalent Ag, 1-DNP-amino-12-biotinamidododecane)2-avidin ((DNP)2-avidin), results 
      in desensitization of a subsequent response, both of the same receptor population 
      (homologous desensitization) and of the second receptor population containing 
      anti-5-dimethylaminonaphthalene-1-sulfonyl (dansyl) IgE (heterologous 
      desensitization). The extent of heterologous desensitization is dependent on 
      several parameters, including the concentration of both the first and the second 
      Ag and the densities of the respective IgE populations. Heterologous 
      desensitization of the Ca2+ response is more sensitive to the concentration of 
      the second stimulus (dansyl-BSA) than heterologous desensitization of the 
      degranulation response. AlF4-, which activates GTP-binding proteins, can 
      effectively replace (DNP)2-avidin as the initial stimulant and desensitizing 
      agent. Other agents that mobilize intracellular Ca2+ including thrombin and a 
      Ca2+ ionophore are less effective at replacing (DNP)2-avidin. Because 
      prestimulation with Ag does not desensitize subsequent responses to AlF4- or Ca2+ 
      ionophore, it appears that signal transduction via Fc epsilon RI is impaired at 
      an early step. Addition of monovalent DNP hapten within approximately 10 min 
      after cross-linking by (DNP)2-avidin completely prevents the desensitization of 
      the subsequent Ca2+ or degranulation response to dansyl-BSA. After longer times 
      of incubation with DNP Ag, the DNP hapten becomes increasingly less effective at 
      preventing the desensitization of the dansyl-BSA response, even though ongoing 
      signal transduction by the DNP Ag is halted. These results suggest a form of 
      cellular memory for the desensitized state.
FAU - Weetall, M
AU  - Weetall M
AD  - Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, NY 
      14853-1301.
FAU - Holowka, D
AU  - Holowka D
FAU - Baird, B
AU  - Baird B
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI22449/AI/NIAID NIH HHS/United States
GR  - GM08267/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Aluminum Compounds)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Receptors, IgE)
RN  - 21340-02-3 (tetrafluoroaluminate)
RN  - 284SYP0193 (Fluorine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - CPD4NFA903 (Aluminum)
RN  - Q80VPU408O (Fluorides)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aluminum/pharmacology
MH  - *Aluminum Compounds
MH  - Animals
MH  - Calcium/metabolism
MH  - Cell Degranulation
MH  - Cell Line
MH  - Dinitrobenzenes/immunology
MH  - *Fluorides
MH  - Fluorine/pharmacology
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/*immunology
MH  - Rats
MH  - Receptors, IgE/*physiology
MH  - Signal Transduction
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1993 May 1;150(9):4072-83.