PMID- 8474118
OWN - NLM
STAT- MEDLINE
DCOM- 19930514
LR  - 20061115
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 38
IP  - 4
DP  - 1993 Apr
TI  - Immunoglobulin-A detection and the investigation of clinical toxoplasmosis.
PG  - 286-92
AB  - Current serological methods for the investigation of Toxoplasma gondii infection 
      are unreliable for the diagnosis of congenital disease, reactivated infection 
      associated with the acquired immune deficiency syndrome (AIDS), or the 
      determination of the date of onset of infection. An enzyme-linked immunosorbent 
      assay (ELISA) and an immunosorbent agglutination assay (ISAGA) were developed for 
      the detection of toxoplasma-specific immunoglobulin-A (IgA) and used to 
      investigate patients in these three categories. The IgA ISAGA and IgA ELISA were 
      found to be reproducible and specific tests. The IgA ISAGA demonstrated enhanced 
      sensitivity. Measurement of IgA in patients with toxoplasma-associated 
      lymphadenopathy of known duration and AIDS patients with toxoplasma infection was 
      of limited value. Detection of specific IgA by ISAGA was more sensitive than 
      conventional methods for the diagnosis of congenital toxoplasmosis. We recommend 
      the investigation of infant sera with IgA ISAGA. IgA estimation is not indicated 
      in other clinical situations.
FAU - Patel, B
AU  - Patel B
AD  - Department of Medical Microbiology, St. George's Hospital, London.
FAU - Young, Y
AU  - Young Y
FAU - Duffy, K
AU  - Duffy K
FAU - Tanner, R P
AU  - Tanner RP
FAU - Johnson, J
AU  - Johnson J
FAU - Holliman, R E
AU  - Holliman RE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin A)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/complications/pathology
MH  - Agglutination Tests/methods
MH  - Animals
MH  - Antibodies, Protozoan/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Humans
MH  - Immunoglobulin A/*analysis
MH  - Immunosorbent Techniques
MH  - Infant
MH  - Lymph Nodes/pathology
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Toxoplasma/*immunology
MH  - Toxoplasmosis/complications/*diagnosis/*immunology/pathology
MH  - Toxoplasmosis, Cerebral/diagnosis/immunology/pathology
MH  - Toxoplasmosis, Congenital/diagnosis/immunology/pathology
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - 10.1099/00222615-38-4-286 [doi]
PST - ppublish
SO  - J Med Microbiol. 1993 Apr;38(4):286-92. doi: 10.1099/00222615-38-4-286.