PMID- 8477797
OWN - NLM
STAT- MEDLINE
DCOM- 19930525
LR  - 20071114
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 23
IP  - 5
DP  - 1993 May
TI  - The fate of human peripheral blood lymphocytes after transplantation into SCID 
      mice.
PG  - 1023-8
AB  - Human peripheral blood lymphocytes (hu-PBL) can be adoptively transferred by 
      intraperitoneal injection into mice with severe combined immunodeficiency (SCID). 
      The transplanted lymphocytes can produce immunoglobulin (Ig), respond to 
      antigens, and survive for months in this chimeric model (hu-PBL SCID). However, 
      whether the lymphocytes actually repopulate and reconstitute lymphoid structures 
      and organs has been subject of some debate. To address this question and to 
      characterize the hu-PBL SCID model better, we employed a novel technique for the 
      identification of human cells in xenogeneic mice. We used fluorescence in situ 
      hybridization (FISH) with a biotinylated DNA probe to all human centromeres. We 
      demonstrated that FISH could be used to detect human cells when they accounted 
      for less than 1% of human/mouse cell mixtures; it could also be employed for the 
      identification and localization of individual human cells in tissue sections. By 
      using FISH, we studied 31 SCID mice injected with 1.5 x 10(7)-4 x 10(7) hu-PBL 
      via intravenous (i.v.) or intraperitoneal (i.p.) routes. In the 6 i.v.-injected 
      mice, we found that the human cells were removed from the circulation into the 
      lung within 1 h. In 22 of 25 i.p.-injected animals, 90-3716 micrograms/ml of 
      human IgG was found in the sera at 3 to 13 weeks after transplantation (a.t.). 
      Human cells colonized the peritoneal cavity and persisted for up to 13 weeks a.t. 
      and, in the 12 mice studied, accounted for 4% to 57% of the cells in the 
      peritoneal fluid. However, only rare, isolated human cells were found in the 
      spleen, blood, bone marrow, lung or Peyer's patches. In 7 of 19 mice that 
      received hu-PBL i.p. from Epstein-Barr virus-seropositive donors, we found masses 
      of human cells usually beneath the peritoneal lining but sometimes infiltrating 
      normal tissue. We conclude that FISH offers a simple means for accurate 
      identification of human cells in the xenogeneic mouse. Although there is 
      colonization of the peritoneal cavity in most mice, and development of lymphoid 
      masses in some, there is no reconstitution of lymphoid structures and only 
      minimal engraftment of lymphoid organs by human cells in conventionally-prepared 
      hu-PBL SCID constructs.
FAU - Martino, G
AU  - Martino G
AD  - Department of Neurology, University of Milano, IRCCS H. San Raffaele, Milano, 
      Italy.
FAU - Anastasi, J
AU  - Anastasi J
FAU - Feng, J
AU  - Feng J
FAU - Mc Shan, C
AU  - Mc Shan C
FAU - DeGroot, L
AU  - DeGroot L
FAU - Quintans, J
AU  - Quintans J
FAU - Grimaldi, L M
AU  - Grimaldi LM
LA  - eng
GR  - R01 AI-19589/AI/NIAID NIH HHS/United States
GR  - R01 CA-19266/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
SB  - IM
MH  - Animals
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Lymphocyte Transfusion
MH  - Lymphocytes/cytology
MH  - Lymphoid Tissue/cytology
MH  - Mice
MH  - Mice, SCID/*immunology
MH  - Peritoneal Cavity/cytology
MH  - *Transplantation, Heterologous
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 10.1002/eji.1830230506 [doi]
PST - ppublish
SO  - Eur J Immunol. 1993 May;23(5):1023-8. doi: 10.1002/eji.1830230506.