PMID- 8477954
OWN - NLM
STAT- MEDLINE
DCOM- 19930524
LR  - 20151119
IS  - 0018-5043 (Print)
IS  - 0018-5043 (Linking)
VI  - 25
IP  - 3
DP  - 1993 Mar
TI  - Mononuclear leukocytes from obese patients with type II diabetes have reduced 
      activity of hexokinase, 6-phosphofructokinase and glucose-6-phosphate 
      dehydrogenase.
PG  - 160-4
AB  - In the present study we measured the activity of some cytosolic enzymes involved 
      in intracellular glucose metabolism in mononuclear leukocytes from 77 obese 
      subjects of which 39 were nondiabetic and 38 had newly-diagnosed untreated type 
      II diabetes mellitus. 28 subjects (19 nondiabetic and 18 diabetic) had also a 
      study of insulin binding to monocytes. 35 subjects (14 nondiabetic, 21 diabetic) 
      underwent an insulin tolerance test for the evaluation of in vivo insulin action. 
      Mononuclear leukocytes from diabetic obese patients showed significantly lower 
      activities of hexokinase (HK), 6-phosphofructokinase (PFK) and 
      glucose-6-phosphate dehydrogenase (G6PDH), while pyruvate kinase (PK) and 
      6-phosphogluconate dehydrogenase (6PGDH) activities were similar in the two 
      groups. In the whole population HK and G6PDH activities inversely correlated with 
      fasting and 2-h OGTT plasma glucose levels. Neither plasma insulin levels nor 
      maximal specific insulin binding to monocytes were significantly correlated with 
      any of the enzyme activities measured. Conversely, the parameter of insulin 
      action generated by insulin tolerance test significantly correlated with HK, 
      G6PDH and 6PGDH. These results indicate that in obese subjects the presence of 
      diabetes is associated with a reduced activity of some enzymes of glucose 
      metabolism in mononuclear leukocytes. This multiple enzymatic defect is 
      correlated with the impairment of in vivo insulin action.
FAU - Muggeo, M
AU  - Muggeo M
AD  - Department of Metabolic Diseases, University of Verona, Italy.
FAU - Moghetti, P
AU  - Moghetti P
FAU - Querena, M
AU  - Querena M
FAU - Cacciatori, V
AU  - Cacciatori V
FAU - Zoppini, G
AU  - Zoppini G
FAU - Zenere, M
AU  - Zenere M
FAU - Tosi, F
AU  - Tosi F
FAU - Travia, D
AU  - Travia D
FAU - Bonora, E
AU  - Bonora E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Horm Metab Res
JT  - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et 
      metabolisme
JID - 0177722
RN  - 0 (Insulin)
RN  - EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Adult
MH  - Diabetes Mellitus/*enzymology
MH  - Diabetes Mellitus, Type 2/*enzymology
MH  - Female
MH  - Glucose Tolerance Test
MH  - Glucosephosphate Dehydrogenase/*blood
MH  - Hexokinase/*blood
MH  - Humans
MH  - Insulin/blood/metabolism
MH  - Leukocytes, Mononuclear/*enzymology
MH  - Male
MH  - Middle Aged
MH  - *Obesity
MH  - Phosphofructokinase-1/*blood
MH  - Phosphogluconate Dehydrogenase/blood
MH  - Pyruvate Kinase/blood
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 10.1055/s-2007-1002068 [doi]
PST - ppublish
SO  - Horm Metab Res. 1993 Mar;25(3):160-4. doi: 10.1055/s-2007-1002068.