PMID- 8485475
OWN - NLM
STAT- MEDLINE
DCOM- 19930604
LR  - 20151119
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 11
IP  - 4
DP  - 1993 Apr
TI  - Detection of residual host cells after bone marrow transplantation using 
      non-isotopic in situ hybridization and karyotype analysis.
PG  - 279-84
AB  - Karyotype analysis and interphase cytogenetics by means of non-radioactive in 
      situ hybridization (NISH) with Y and X-specific probes were used to detect 
      residual host cells in BM of 18 male patients who had received a BMT from their 
      sisters. All patients but one had a malignant blood disease; 17 patients were 
      clinically in continuous remission at the time of the investigations and 
      throughout the follow-up period. No host cells were detected by karyotype 
      analysis in patients who were in clinical remission. NISH with a biotinylated 
      Y-specific probe showed residual host cells in 16 of the 17 patients in clinical 
      remission. In samples from three patients the existence of host cells was further 
      confirmed by revealing the XY-containing cells with two-color fluorescence in 
      situ hybridization (FISH) using X- and Y-specific probes on direct BM smears 
      showed > 3% residual host cells in 50% of the samples studied at or later than 2 
      months post-BMT. On conventional cytogenetic preparations < 1% Y-specific cells 
      were detected in all but two samples at > or = 2 months post-BMT. There was no 
      difference in the proportion of host cells between patients conditioned with 
      total body irradiation and CY and those who received busulphan and CY. The 
      recipient's stromal or epithelial cells in the aspirates probably account for 
      most of the host cells detected. In conclusion, small numbers of residual host 
      cells detected by interphase cytogenetics with a Y-specific probe do not indicate 
      an imminent relapse.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Wessman, M
AU  - Wessman M
AD  - Department of Medical Genetics, University of Helsinki, Finland.
FAU - Popp, S
AU  - Popp S
FAU - Ruutu, T
AU  - Ruutu T
FAU - Volin, L
AU  - Volin L
FAU - Cremer, T
AU  - Cremer T
FAU - Knuutila, S
AU  - Knuutila S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers/analysis
MH  - Bone Marrow/*pathology
MH  - Bone Marrow Purging
MH  - Bone Marrow Transplantation/mortality/*pathology
MH  - Cell Survival
MH  - *Chimera
MH  - Female
MH  - Graft Survival
MH  - Humans
MH  - *In Situ Hybridization
MH  - *Karyotyping
MH  - Leukemia/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Nuclear Family
MH  - Remission Induction
MH  - Transplantation, Homologous
MH  - Treatment Outcome
MH  - Y Chromosome
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PST - ppublish
SO  - Bone Marrow Transplant. 1993 Apr;11(4):279-84.