PMID- 8489281
OWN - NLM
STAT- MEDLINE
DCOM- 19930607
LR  - 20061115
IS  - 0385-0684 (Print)
IS  - 0385-0684 (Linking)
VI  - 20
IP  - 6
DP  - 1993 Apr
TI  - [Detection of chromosomal aberration using fluorescence in situ hybridization in 
      DNA diploid colorectal carcinomas].
PG  - 759-62
AB  - Numerical aberrations of chromosomes can be detected by fluorescence in situ 
      hybridization (FISH), using chromosome-specific probes. It is possible to observe 
      this in solid tumors from which it is very difficult to obtain metaphase nuclei. 
      The present study employed surgical specimens from 15 cases of colorectal 
      carcinoma, all of which showed DNA diploidy. In the same samples, we analyzed 
      chromosomal numerical aberration by FISH according to the method of Pinkel et al. 
      Biotinylated DNA probes specific to chromosome #7, #11 and #17, were used. The 
      hybridization spots were observed by fluorescent microscopy. As a result, the 
      numerical aberrations of chromosomes detected by FISH were found in DNA diploid 
      cases by FCM. They were trisomy and monosomy. These results indicate that FISH is 
      useful to detect the chromosomal aberrations of DNA diploid cases.
FAU - Jibiki, M
AU  - Jibiki M
AD  - First Dept. of Surgery, Nagasaki University School of Medicine.
FAU - Tagawa, Y
AU  - Tagawa Y
FAU - Miyashita, K
AU  - Miyashita K
FAU - Hara, S
AU  - Hara S
FAU - Yasutake, T
AU  - Yasutake T
FAU - Nakazaki, T
AU  - Nakazaki T
FAU - Obatake, M
AU  - Obatake M
FAU - Sawai, T
AU  - Sawai T
FAU - Morinaga, M
AU  - Morinaga M
FAU - Akama, F
AU  - Akama F
AU  - et al.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Gan To Kagaku Ryoho
JT  - Gan to kagaku ryoho. Cancer & chemotherapy
JID - 7810034
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 7
MH  - Colorectal Neoplasms/*genetics
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Ploidies
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PST - ppublish
SO  - Gan To Kagaku Ryoho. 1993 Apr;20(6):759-62.