PMID- 8490716
OWN - NLM
STAT- MEDLINE
DCOM- 19930614
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 606
IP  - 2
DP  - 1993 Mar 26
TI  - Perfusate serotonin increases extracellular dopamine in the nucleus accumbens as 
      measured by in vivo microdialysis.
PG  - 195-9
AB  - The effects of local application of serotonin (5-HT) on extracellular levels of 
      dopamine (DA) in the nucleus accumbens (N. ACC) were assessed using in vivo 
      microdialysis. At a perfusate flow rate of 0.3 microliter/min the baseline 
      dialysate concentration of DA was 2.1 +/- 0.7 nM (mean +/- S.E.M.; n = 5) and 
      significantly increased to 142 +/- 18%, 220 +/- 47% and 332 +/- 35% of baseline 
      when 0.1 microM, 0.2 microM and 0.4 microM concentrations of 5-HT were included 
      in the perfusate. Perfusate 5-HT concentrations below 0.1 microM had no effect on 
      dialysate DA. The in vivo dialysis efficiency for 5-HT was found to be 39 +/- 
      12%, and thus the concentrations of 5-HT reaching the extracellular space at the 
      surface of the dialysis membrane were estimated to be 40, 80 and 160 nM for the 
      0.1, 0.2 and 0.4 microM 5-HT perfusates, respectively. The serotonin-induced 
      increase in dialysate DA was attenuated by co-perfusion of 0.4 microM 5-HT with 4 
      microM concentrations of pindolol (a relatively non-specific 5-HT1 antagonist; 
      151 +/- 7% vs. 332 +/- 35% baseline dialysate DA for 5-HT/antagonist and 
      5-HT-only perfusates, respectively), LY 53,857 (a specific 5-HT2 antagonist; 130 
      +/- 17% vs. 332 +/- 35%) and MDL 7222 (a specific 5-HT3 antagonist; 143 +/- 19% 
      vs. 332 +/- 35%).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Parsons, L H
AU  - Parsons LH
AD  - Emory University, Department of Chemistry, Atlanta, GA 30322.
FAU - Justice, J B Jr
AU  - Justice JB Jr
LA  - eng
GR  - DA-07532/DA/NIDA NIH HHS/United States
GR  - K02 DA 00179/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Ergolines)
RN  - 0 (Serotonin Antagonists)
RN  - 0 (Tropanes)
RN  - 102-32-9 (3,4-Dihydroxyphenylacetic Acid)
RN  - 333DO1RDJY (Serotonin)
RN  - BJ4HF6IU1D (Pindolol)
RN  - L37TVX7U5M (LY 53857)
RN  - O98T3677PA (bemesetron)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 3,4-Dihydroxyphenylacetic Acid/metabolism
MH  - Animals
MH  - Dialysis/methods
MH  - Dopamine/*metabolism
MH  - Ergolines/pharmacology
MH  - Extracellular Matrix/*metabolism
MH  - Male
MH  - Nucleus Accumbens/*metabolism
MH  - Osmolar Concentration
MH  - Perfusion
MH  - Pindolol/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Serotonin/*pharmacology
MH  - Serotonin Antagonists/pharmacology
MH  - Tropanes/pharmacology
EDAT- 1993/03/26 00:00
MHDA- 2001/03/28 10:01
CRDT- 1993/03/26 00:00
PHST- 1993/03/26 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/03/26 00:00 [entrez]
AID - 0006-8993(93)90984-U [pii]
AID - 10.1016/0006-8993(93)90984-u [doi]
PST - ppublish
SO  - Brain Res. 1993 Mar 26;606(2):195-9. doi: 10.1016/0006-8993(93)90984-u.