PMID- 8493867
OWN - NLM
STAT- MEDLINE
DCOM- 19930616
LR  - 20190905
IS  - 0001-6632 (Print)
IS  - 0001-6632 (Linking)
VI  - 43
IP  - 4
DP  - 1993 Apr
TI  - Collagen synthesis of human arterial smooth muscle cells: effects of 
      platelet-derived growth factor, transforming growth factor-beta 1 and 
      interleukin-1.
PG  - 160-7
AB  - The effects of platelet-derived growth factor (PDGF), transforming growth 
      factor-beta 1 (TGF-beta 1) and interleukin-1 (IL-1) on collagen synthesis of 
      cultured human arterial smooth muscle cells in a confluent state were 
      investigated. Synthetic activity of collagenous protein was determined with 
      [3H]-proline uptake, and subsequent analysis of collagen types by sodium 
      dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by 
      fluorography. Although PDGF (0.5 U/mL and 5.0 U/mL) enhanced total collagen 
      synthesis per dish, it suppressed total collagen synthesis per DNA (DNA content 
      in a dish). TGF-beta 1 (10 pmol/L and 100 pmol/L) enhanced total collagen 
      synthesis both per dish and per DNA. IL-1 (0.1 U/mL and 1.0 U/mL) suppressed 
      total collagen synthesis both per dish and per DNA. A fluorogram revealed that 
      human arterial smooth muscle cells synthesize types I, III, IV and V collagen. 
      Densitometric analysis showed PDGF suppressed the proportion of type IV collagen 
      and increased that of type V collagen. TGF-beta 1 increased the proportions of 
      types IV and V collagen. IL-1 elicited un- remarkable change in the proportion of 
      collagen types. These results suggest that, in the event of human 
      atherosclerosis, TGS-beta 1 is most effective in enhancing collagen synthesis, 
      and PDGF modulates collagen metabolism by stimulating a cell division of smooth 
      muscle cells with a resultant increase of collagenous protein, especially of type 
      V collagen.
FAU - Okada, Y
AU  - Okada Y
AD  - Department of Pathology, School of Medicine, Kanazawa University, Japan.
FAU - Katsuda, S
AU  - Katsuda S
FAU - Watanabe, H
AU  - Watanabe H
FAU - Nakanishi, I
AU  - Nakanishi I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Acta Pathol Jpn
JT  - Acta pathologica japonica
JID - 0372637
RN  - 0 (Interleukin-1)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aorta
MH  - Cells, Cultured
MH  - Collagen/*biosynthesis/drug effects
MH  - DNA/analysis
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Male
MH  - Muscle, Smooth, Vascular/drug effects/*metabolism
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1993.tb01127.x [doi]
PST - ppublish
SO  - Acta Pathol Jpn. 1993 Apr;43(4):160-7. doi: 10.1111/j.1440-1827.1993.tb01127.x.