PMID- 8495362
OWN - NLM
STAT- MEDLINE
DCOM- 19930621
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 608
IP  - 2
DP  - 1993 Apr 16
TI  - Stimulation of tumor necrosis factor-alpha production by mycoplasmas and 
      inhibition by dexamethasone in cultured astrocytes.
PG  - 273-9
AB  - Elevated levels of tumor necrosis factor-alpha (TNF alpha) and other cytokines 
      and eicosanoids in the central nervous system (CNS) have been noted in several 
      human neurologic diseases, including multiple sclerosis and AIDS dementia. 
      Recently it was shown that glial cells, especially astrocytes, are a major source 
      of cytokines and eicosanoids. In the present study we have shown that astrocytes 
      derived from fetal rat brain triggered by mycoplasmas produce TNF alpha and 
      prostaglandin E2 (PGE2). Addition of mycoplasma (Mycoplasma capricolum isolated 
      from sheep and M. fermentans KL-4 from human) at a concentration of 1-50 
      micrograms protein/ml (2 x 10(7)-10(9) colony forming units/ml), as well as 
      lipopolysaccharide (5 micrograms/ml), led to a 200-500-fold increase in TNF alpha 
      and a 2.5-4.5-fold increase in PGE2 production. Preincubation of the cells with 
      the synthetic glucocorticoid, dexamethasone (2 x 10(-5)-2 x 10(-8) M), as well as 
      with the natural hormone, corticosterone, markedly inhibited the secretion of 
      both TNF alpha and PGE2. Thus, mycoplasmas can be added to the wide variety of 
      agents that stimulate glial cells to produce cytokines and eicosanoids, and may 
      contribute to various CNS pathological manifestations. In addition, the ability 
      of glucocorticoids to inhibit particularly the stimulated productions of TNF 
      alpha and PGE2 may explain at least in part the therapeutic benefit of these 
      agents in CNS inflammation and demyelination.
FAU - Brenner, T
AU  - Brenner T
AD  - Department of Neurology, Hadassah University Hospital, Jerusalem, Israel.
FAU - Yamin, A
AU  - Yamin A
FAU - Abramsky, O
AU  - Abramsky O
FAU - Gallily, R
AU  - Gallily R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Astrocytes/drug effects/*metabolism
MH  - Corticosterone/pharmacology
MH  - Dexamethasone/*pharmacology
MH  - Dinoprostone/immunology/*metabolism
MH  - Female
MH  - Lipopolysaccharides/pharmacology
MH  - Mycoplasma/*metabolism
MH  - Mycoplasma fermentans/metabolism
MH  - Neuroglia/drug effects/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/immunology
EDAT- 1993/04/16 00:00
MHDA- 1993/04/16 00:01
CRDT- 1993/04/16 00:00
PHST- 1993/04/16 00:00 [pubmed]
PHST- 1993/04/16 00:01 [medline]
PHST- 1993/04/16 00:00 [entrez]
AID - 0006-8993(93)91468-8 [pii]
AID - 10.1016/0006-8993(93)91468-8 [doi]
PST - ppublish
SO  - Brain Res. 1993 Apr 16;608(2):273-9. doi: 10.1016/0006-8993(93)91468-8.