PMID- 8496391
OWN - NLM
STAT- MEDLINE
DCOM- 19930623
LR  - 20190501
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 46
IP  - 4
DP  - 1993 Apr
TI  - Cytokine gene expression in skin and lymphoid organs in graft versus host 
      disease.
PG  - 341-5
AB  - AIM: To determine if human graft versus host disease (GvHD) is associated with 
      any detectable change in cytokine gene expression in the skin and lymphoid 
      organs. METHODS: Reverse transcriptase and the polymerase chain reaction were 
      used to amplify mRNA for interleukins-1 (IL-1), -2 (IL-2), -4 (IL-4) and -6 
      (IL-6), IL-2 receptor (IL-2R), tumour necrosis factors alpha (TNF-alpha) and beta 
      (TNF-beta), gamma interferon (IFN gamma) and granulocyte macrophage colony 
      stimulating factor (GM-CSF) in frozen punch biopsy specimens of skin and necropsy 
      samples of skin, lymph node, and spleen. RESULTS: No cytokine mRNA was detected 
      in the punch biopsy specimens except weak signals for IL-6 and IL-1 and GM-CSF in 
      two normal donors and IL2-R in one patient with GvHD. In samples of skin taken at 
      necropsy, however, significant quantities of mRNA for TNF-alpha, TNF-beta, and 
      IL-4 were detected in patients who had or had had GvHD in contrast to those 
      without the disease whose skin lacked mRNA for these products but contained 
      detectable quantities of IL-1, IL2-R, IL-6 and GM-CSF. There seemed to be a 
      reciprocal relation between TNF-alpha and IL-4. In necropsy samples of lymph node 
      and spleen a pattern of cytokine production similar to that in the skin was 
      observed with a preponderance of TNF-alpha, TNF-beta and IL-4 in patients with 
      GvHD and GM-CSF and IL-6 in those without the disease. CONCLUSIONS: The local 
      synthesis of these molecules would explain many of the morphological and 
      immunohistological features of GvHD. The failure to detect TNF-alpha, TNF-beta, 
      and IL-4 in skin biopsy specimens exhibiting GvHD is probably due to their small 
      size but further investigations are required.
FAU - Rowbottom, A W
AU  - Rowbottom AW
AD  - Department of Immunology, Charing Cross and Westminster Medical School, London.
FAU - Norton, J
AU  - Norton J
FAU - Riches, P G
AU  - Riches PG
FAU - Hobbs, J R
AU  - Hobbs JR
FAU - Powles, R L
AU  - Powles RL
FAU - Sloane, J P
AU  - Sloane JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Cytokines)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Child
MH  - Cytokines/*genetics
MH  - DNA/chemistry
MH  - Female
MH  - Gene Expression/*physiology
MH  - Graft vs Host Disease/*genetics/immunology
MH  - Humans
MH  - Lymph Nodes/immunology
MH  - Lymphoid Tissue/*immunology
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Skin/*immunology
MH  - Spleen/immunology
PMC - PMC501216
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
PMCR- 1996/04/01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
PHST- 1996/04/01 00:00 [pmc-release]
AID - 10.1136/jcp.46.4.341 [doi]
PST - ppublish
SO  - J Clin Pathol. 1993 Apr;46(4):341-5. doi: 10.1136/jcp.46.4.341.