PMID- 8496603
OWN - NLM
STAT- MEDLINE
DCOM- 19930622
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 150
IP  - 11
DP  - 1993 Jun 1
TI  - cDNA cloning of guinea pig monocyte chemoattractant protein-1 and expression of 
      the recombinant protein.
PG  - 5025-32
AB  - Monocyte chemoattractant protein-1 (MCP-1) cDNA was cloned from guinea pig spleen 
      cells stimulated with Con A. The cDNA comprised 647 bp with an open reading frame 
      that encoded a 120 amino acid protein. The sequence similarity of the first 99 
      amino acids to human MCP-1 is 56%. Recombinant guinea pig MCP-1 was expressed in 
      COS-7 cells, then purified by a three step procedure with orange A-agarose, 
      carboxymethyl-HPLC, and reversed phase-HPLC. The purified protein was found 
      around 25 kDa as a broad band on a polyacrylamide gel under reducing conditions. 
      Guinea pig MCP-1 attracted about 34% of input human monocytes at 5 x 10(-9) M. 
      Guinea pig peritoneal exudate macrophages migrated toward guinea pig MCP-1 dose 
      dependently, but only 1% of input cells responded to guinea pig MCP-1 at its 
      optimal concentration of 5 x 10(-9) M. Human MCP-1 attracted 1% of input guinea 
      pig peritoneal exudate macrophages at its highest concentration of 2.5 x 10(-8) 
      M. Neither human nor guinea pig MCP-1 attracted guinea pig peritoneal resident 
      macrophages. These results suggest that monocytes lose responsiveness to MCP-1 
      after differentiating to macrophages. Finally, intradermal injection of the 
      recombinant protein into guinea pigs caused marked macrophage infiltration. 
      Cloned and expressed guinea pig MCP-1 will help in studying the role of MCP-1 in 
      vivo.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute-Frederick Cancer Research and 
      Development Center, MD 21702.
LA  - eng
SI  - GENBANK/L04985
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/biosynthesis/*genetics/pharmacology
MH  - Cloning, Molecular
MH  - Cytokines/*genetics
MH  - DNA/genetics
MH  - Guinea Pigs
MH  - Humans
MH  - Injections, Intradermal
MH  - *Macrophages
MH  - Molecular Sequence Data
MH  - Monocytes/drug effects/immunology
MH  - Recombinant Proteins/pharmacology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1993 Jun 1;150(11):5025-32.