PMID- 8497063
OWN - NLM
STAT- MEDLINE
DCOM- 19930621
LR  - 20201209
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 6
DP  - 1993 Jun
TI  - cis-acting requirements for the replication of flock house virus RNA 2.
PG  - 3544-51
AB  - To examine the cis-acting requirements for RNA replication, a cDNA clone of flock 
      house virus (FHV) RNA 2 was transfected into baby hamster kidney cells and 
      transcribed to yield RNAs that had terminal extensions of different lengths or 
      that lacked internal regions of the molecule. These RNAs were tested for their 
      ability to be replicated by FHV replicase that was provided by cotransfection of 
      purified FHV RNA 1. The results showed that RNA replication was inhibited by 
      terminal extensions, particularly those at the 5' end of the RNA, despite the 
      fact that these extensions were corrected during RNA replication. A 
      negative-sense transcript with a 12-nucleotide 3' extension was replicated to 
      produce a positive-sense RNA that had the correct 5' end, showing that the 
      replicase could select its correct initiation site from within a longer template. 
      A uridine residue at the second position of the positive strand was an important 
      determinant of template activity. RNA molecules with large internal deletions 
      that amounted to almost 50% of the 1,400 nucleotides of RNA 2 replicated as 
      efficiently as full-length molecules, but only if they contained an internal 
      region that lay between nucleotides 538 and 616. Thirty-six spontaneous deletions 
      of RNA 2 that arose during sequential replicative passages all conserved the same 
      internal region of the molecule. These results establish that both terminal and 
      internal regions of FHV RNA 2 play essential roles in making the molecule a 
      competent template for replication.
FAU - Ball, L A
AU  - Ball LA
AD  - Department of Microbiology, University of Alabama, Birmingham 35294-0005.
FAU - Li, Y
AU  - Li Y
LA  - eng
GR  - R37 AI18270/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Insect Viruses/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Plasmids/genetics
MH  - Polymerase Chain Reaction
MH  - RNA Viruses/*genetics/metabolism
MH  - RNA, Viral/*biosynthesis
MH  - RNA-Dependent RNA Polymerase/metabolism
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Sequence Deletion
MH  - Substrate Specificity
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC237701
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
PMCR- 1993/06/01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.6.3544-3551.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Jun;67(6):3544-51. doi: 10.1128/JVI.67.6.3544-3551.1993.