PMID- 8498524
OWN - NLM
STAT- MEDLINE
DCOM- 19930622
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 264
IP  - 5 Pt 1
DP  - 1993 May
TI  - Effect of mode of activation of human eosinophils on tracheal smooth muscle 
      contraction in guinea pigs.
PG  - L475-81
AB  - We studied the relationship between mode of activation of isolated human 
      eosinophils and in situ responsiveness in isolated tracheal smooth muscle (TSM) 
      of guinea pigs. Human peripheral blood eosinophils were activated with either 
      10(-7) M phorbol myristate acetate (PMA) or 10(-6) M 
      formyl-methionyl-leucyl-phenylalanine (fMLP) + 5 micrograms/ml cytochalasin B 
      (CYB), and activation was confirmed by measurement of eosinophil peroxidase (EPO) 
      secretion by kinetic assay. EPO secretion was similar after activation with 
      fMLP+CYB (10.2 +/- 3.2% of total eosinophil content) and PMA (10.0 +/- 2.8% of 
      total content; P = NS). Topical application of 6 x 10(6) eosinophils/cm2 
      activated with fMLP+CYB to the TSM segment caused 0.51 +/- 0.14 g/cm active 
      tension (AT) in five preparations (P < 0.03 vs. baseline); cells activated with 
      PMA caused no contractile response (0.04 +/- 0.03 g/cm AT, P = NS vs. baseline). 
      Both PMA- and fMLP+CYB-activated cells caused augmentation of muscarinic 
      responsiveness of guinea pig trachealis. The dose of intravenous acetylcholine 
      required to cause a threshold response (ED0.3) was -7.3 +/- 0.1 log mol/kg at 
      baseline vs. -8.7 +/- 0.5 log mol/kg after treatment with fMLP+CYB-activated 
      eosinophils (P = 0.05) and -6.9 +/- 0.1 log mol/kg at baseline vs. -7.5 +/- 0.1 
      log mol/kg after PMA-activated cells (P < 0.01). Both AT and augmented muscarinic 
      responsiveness were blocked by pretreating the eosinophils with 200 microM 
      A-63162, an inhibitor of 5-lipoxygenase, before activation with fMLP+CYB. We 
      demonstrate that eosinophils activated comparably (as assessed by EPO secretion) 
      cause augmented muscarinic responsiveness and/or direct contraction of guinea pig 
      TSM through secretion of a product of the 5-lipoxygenase pathway.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Strek, M E
AU  - Strek ME
AD  - Department of Medicine, University of Chicago, Illinois 60637.
FAU - White, S R
AU  - White SR
FAU - Hsiue, T R
AU  - Hsiue TR
FAU - Kulp, G V
AU  - Kulp GV
FAU - Williams, F S
AU  - Williams FS
FAU - Leff, A R
AU  - Leff AR
LA  - eng
GR  - HL-02484/HL/NHLBI NIH HHS/United States
GR  - HL-32495/HL/NHLBI NIH HHS/United States
GR  - HL-46368/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Acetamides)
RN  - 0 (Lipoxygenase Inhibitors)
RN  - 0 (Phenyl Ethers)
RN  - 0452Y37YTA (N-hydroxy-N-(1-(4-(phenylmethoxy)phenyl)ethyl)-acetamide)
RN  - 11096-26-7 (Erythropoietin)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - N9YNS0M02X (Acetylcholine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Acetamides/pharmacology
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Cytochalasin B/pharmacology
MH  - Eosinophils/drug effects/*physiology
MH  - Erythropoietin/blood/metabolism
MH  - Guinea Pigs
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Lipoxygenase Inhibitors/pharmacology
MH  - Male
MH  - *Muscle Contraction
MH  - Muscle, Smooth/*physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Phenyl Ethers
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Trachea/*physiology
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 10.1152/ajplung.1993.264.5.L475 [doi]
PST - ppublish
SO  - Am J Physiol. 1993 May;264(5 Pt 1):L475-81. doi: 10.1152/ajplung.1993.264.5.L475.