PMID- 8499346
OWN - NLM
STAT- MEDLINE
DCOM- 19930625
LR  - 20190830
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 45
IP  - 5
DP  - 1993 May
TI  - Characterization of 11 beta-hydroxysteroid dehydrogenase of human placenta: 
      evidence for the existence of two species of 11 beta-hydroxysteroid 
      dehydrogenase.
PG  - 391-7
AB  - The enzyme, 11 beta-hydroxysteroid dehydrogenase converts the active 
      glucocorticoids cortisol and corticosterone to their inactive 11-oxo metabolites 
      cortisone and dehydrocorticosterone, respectively. The properties of the human 
      placental 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) were studied. The 
      enzyme was active in the oxidative and reductive directions. pH optimum for 11 
      beta-dehydrogenase activity was in the range of 7-10 and for 11-oxoreductase it 
      was in the range of 5.5-6.0. The crude placental homogenate was unstable. 
      Reductase activity was more labile than dehydrogenase activity. Removal of 
      cytosol enabled the enzyme to retain activity. 11 beta-HSD a membrane bound 
      enzyme was distributed in all particulate subcellular fractions. Addition of 
      detergent released latent activity of 11 beta-dehydrogenase and inactivated 
      11-reductase activity. Both corticosterone and cortisol were substrates for the 
      enzyme. The Km value with corticosterone as substrate was much lower than with 
      cortisol. The Km values with cortisone and dehydrocorticosterone were similar.
FAU - Lakshmi, V
AU  - Lakshmi V
AD  - Department of Obstetrics and Gynecology, SUNY Health Science Center, Brooklyn 
      11203.
FAU - Nath, N
AU  - Nath N
FAU - Muneyyirci-Delale, O
AU  - Muneyyirci-Delale O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - W980KJ009P (Corticosterone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Cell Membrane/enzymology
MH  - Cell Nucleus/enzymology
MH  - Corticosterone/metabolism
MH  - Enzyme Stability
MH  - Female
MH  - Humans
MH  - Hydrocortisone/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Kinetics
MH  - Microsomes/enzymology
MH  - Mitochondria/enzymology
MH  - NAD/pharmacology
MH  - Oxidation-Reduction
MH  - Placenta/*enzymology/ultrastructure
MH  - Pregnancy
MH  - Substrate Specificity
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 10.1016/0960-0760(93)90008-k [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 1993 May;45(5):391-7. doi: 
      10.1016/0960-0760(93)90008-k.