PMID- 8499638
OWN - NLM
STAT- MEDLINE
DCOM- 19930701
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 81
IP  - 11
DP  - 1993 Jun 1
TI  - Variability of 11q23 rearrangements in hematopoietic cell lines identified with 
      fluorescence in situ hybridization.
PG  - 3027-33
AB  - We mapped and ordered 17 cosmid, phage, and plasmid clones to chromosome 11, 
      bands q22-q24, using fluorescence in situ hybridization (FISH). We then analyzed 
      four hematopoietic cell lines with 11q23 rearrangements, Karpas 45, SUP-T13, 
      RC-K8, and Karpas 422, using these probes. The studies showed that the 
      translocation breakpoints of the Karpas 45 and SUP-T13 cell lines, which were 
      derived from T-cell malignancies, were located in the same breakpoint cluster 
      region of the MLL gene as the RS4; 11 cell line and patients with the t(9;11), 
      t(11;19), and t(6;11) described previously. We confirmed that the translocation 
      breakpoint of the RC-K8 cell line was located telomeric to the MLL gene, and 
      found that the derivative 11 chromosome of the Karpas 422 cell line, which had 
      been thought to contain a t(4;11) (q21;q23), was in fact formed through a 
      deletion and an inverted tandem repeat of part of 11q.
FAU - Kobayashi, H
AU  - Kobayashi H
AD  - Department of Medicine, University of Chicago, IL 60637.
FAU - Espinosa, R 3rd
AU  - Espinosa R 3rd
FAU - Thirman, M J
AU  - Thirman MJ
FAU - Davis, E M
AU  - Davis EM
FAU - Diaz, M O
AU  - Diaz MO
FAU - Le Beau, M M
AU  - Le Beau MM
FAU - Rowley, J D
AU  - Rowley JD
LA  - eng
GR  - CA38725/CA/NCI NIH HHS/United States
GR  - CA40046/CA/NCI NIH HHS/United States
GR  - CA42577/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Probes)
SB  - IM
GS  - CD3D
GS  - EST1
GS  - NCAM
GS  - PBGD
GS  - SPRP
GS  - THY1
MH  - Cell Line
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - DNA Probes
MH  - Gene Rearrangement
MH  - Hematopoietic Stem Cells/*ultrastructure
MH  - Humans
MH  - In Situ Hybridization
MH  - Translocation, Genetic
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - S0006-4971(20)71545-4 [pii]
PST - ppublish
SO  - Blood. 1993 Jun 1;81(11):3027-33.