PMID- 8499642
OWN - NLM
STAT- MEDLINE
DCOM- 19930701
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 81
IP  - 11
DP  - 1993 Jun 1
TI  - gamma-Interferon in multiple myeloma: inhibition of interleukin-6 
      (IL-6)-dependent myeloma cell growth and downregulation of IL-6-receptor 
      expression in vitro.
PG  - 3076-82
AB  - In multiple myeloma, malignant plasma cells from most patients with active 
      disease proliferate spontaneously when cultured for 5 days in vitro. This 
      spontaneous proliferation is related to the endogenous production of 
      interleukin-6 (IL-6), the major myeloma-cell growth factor. A 50% inhibitory dose 
      (100 U/mL) of human recombinant gamma-interferon (hr gamma-IFN) blocked the 
      proliferation of myeloma cells almost completely in all 19 patients analyzed. 
      This inhibition was not caused by suppression of endogenous IL-6 production and 
      was also observed in the presence of an excess of hrIL-6. hr gamma-IFN was also 
      completely inhibitory in four human myeloma cell lines (HMCL) whose growth is 
      totally dependent on the addition of exogenous hrIL-6. This inhibition was 
      associated with a 47% to 73% decrease in membrane IL-6-binding gp80 protein as 
      well as with a 90% decrease in the amount of gp80 mRNA in HMCL. These results are 
      in line with recent reports indicating that gamma-IFN inhibited several 
      IL-6-dependent biologic processes. They suggest a need to reconsider why previous 
      preliminary clinical trials failed to demonstrate a beneficial effect of 
      gamma-IFN in multiple myeloma.
FAU - Portier, M
AU  - Portier M
AD  - INSERM U291, Immunopathologie des Maladies Tumorales et Autoimmunes, Montpellier, 
      France.
FAU - Zhang, X G
AU  - Zhang XG
FAU - Caron, E
AU  - Caron E
FAU - Lu, Z Y
AU  - Lu ZY
FAU - Bataille, R
AU  - Bataille R
FAU - Klein, B
AU  - Klein B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Bone Marrow/pathology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon-gamma/*pharmacology/therapeutic use
MH  - Interleukin-6/biosynthesis
MH  - Leukemia, Plasma Cell/pathology
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Multiple Myeloma/*drug therapy/pathology
MH  - RNA, Messenger/genetics
MH  - Receptors, Immunologic/genetics/metabolism
MH  - Receptors, Interleukin-6
MH  - Recombinant Proteins
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - S0006-4971(20)71551-X [pii]
PST - ppublish
SO  - Blood. 1993 Jun 1;81(11):3076-82.