PMID- 8499685
OWN - NLM
STAT- MEDLINE
DCOM- 19930629
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 10
IP  - 1
DP  - 1993 Jan
TI  - A fast and efficient method for simultaneous X and Y in situ hybridization of 
      human blastomeres.
PG  - 82-90
AB  - PURPOSE: To use a 6-hr fluorescence in situ hybridization (FISH) procedure 
      involving fluorochrome-labeled probes to determine the gender of blastomeres from 
      arrested biopsied human embryos. RESULTS: Simultaneous detection of X and Y 
      chromosomes was performed on 68 blastomeres with this technique. The FISH 
      efficiency for gender determination was 95.5% (65/68). In addition, 
      rehybridization with chromosome 8-specific probes was performed to determine the 
      ploidy of blastomeres with more than two sex chromosomes. CONCLUSION: This 
      technique offers an alternative to polymerase chain reaction for the 
      preimplantation diagnosis of X-linked diseases and can also be used for ploidy 
      assessment.
FAU - Munne, S
AU  - Munne S
AD  - Gamete and Embryo Research Laboratory, New York Hospital-Cornell University 
      Medical Center, New York 10021.
FAU - Weier, H U
AU  - Weier HU
FAU - Stein, J
AU  - Stein J
FAU - Grifo, J
AU  - Grifo J
FAU - Cohen, J
AU  - Cohen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Fixatives)
SB  - IM
MH  - Biopsy
MH  - Blastomeres/pathology/*physiology
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Disorders
MH  - Fixatives
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Ploidies
MH  - Reproducibility of Results
MH  - Sex Determination Analysis/*methods
MH  - Time Factors
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF01204446 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1993 Jan;10(1):82-90. doi: 10.1007/BF01204446.