PMID- 8504147
OWN - NLM
STAT- MEDLINE
DCOM- 19930706
LR  - 20170915
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1168
IP  - 2
DP  - 1993 Jun 12
TI  - Glycosphingolipid compositions of human T-lymphotropic virus type I (HTLV-I) and 
      human immunodeficiency virus (HIV)-infected cell lines.
PG  - 123-9
AB  - Glycolipid compositions of cells infected by human retroviruses (human 
      immunodeficiency virus, HIV and/or human T-cell lymphotropic virus type I, 
      HTLV-I) have been studied. Eight cell lines, comprising two HTLV-I-infected 
      T-cell lines (MT-2 and MT-4), two HTLV-I-negative T-cell lines (Jurkat and MF), a 
      macrophage cell line (U937), and three HIV-infected counterpart cell lines 
      (MT-4/HIV, Jurkat/HIV and U937/HIV) were used. The neutral glycolipids and 
      gangliosides isolated from these cell lines were compared. Among them, the 
      HTLV-I-infected T-cell lines, MT-2 and MT-4, showed similar patterns for both 
      neutral glycolipids and gangliosides. Neutral glycolipids (GlcCer and LacCer) of 
      MT-2 and MT-4 cells were markedly decreased, and a ganglioside, GM3, of theirs 
      was decreased to only a trace amount compared to that in other cell lines. 
      Gangliosides of MT-4 and MT-4/HIV were further separated on an Iatrobeads column, 
      and were identified as GM2, GM1a and GD1a by methylation and liquid secondary ion 
      mass spectrometric analyses. Since the patterns of neutral glycolipids and 
      gangliosides of MT-2 and MT-4 are unique, as compared to those of HTLV-I-negative 
      cells, it is suggested that these changes are related to HTLV-1 infection. No 
      prominent differences in the ganglioside compositions between HIV-infected and 
      non-infected cell lines could be observed. But it is noteworthy that the contents 
      of asialo-GM2 in Jurkat/HIV and MT-4/HIV cells were increased as compared to 
      those in the parental cell lines.
FAU - Matsuda, K
AU  - Matsuda K
AD  - Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental 
      University, Japan.
FAU - Taki, T
AU  - Taki T
FAU - Hamanaka, S
AU  - Hamanaka S
FAU - Kasama, T
AU  - Kasama T
FAU - Rokukawa, C
AU  - Rokukawa C
FAU - Handa, S
AU  - Handa S
FAU - Yamamoto, N
AU  - Yamamoto N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Gangliosides)
RN  - 0 (Glycosphingolipids)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Carbohydrate Sequence
MH  - Cell Line/metabolism/microbiology
MH  - Chromatography, Thin Layer
MH  - Gangliosides/analysis/chemistry
MH  - Glycosphingolipids/*analysis/isolation & purification
MH  - HIV Infections/*metabolism
MH  - *HIV-1
MH  - HTLV-I Infections/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neuraminidase
EDAT- 1993/06/12 00:00
MHDA- 1993/06/12 00:01
CRDT- 1993/06/12 00:00
PHST- 1993/06/12 00:00 [pubmed]
PHST- 1993/06/12 00:01 [medline]
PHST- 1993/06/12 00:00 [entrez]
AID - 0005-2760(93)90116-Q [pii]
PST - ppublish
SO  - Biochim Biophys Acta. 1993 Jun 12;1168(2):123-9.