PMID- 8504397
OWN - NLM
STAT- MEDLINE
DCOM- 19930706
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 67
IP  - 1
DP  - 1993 May
TI  - Transfected c-Ha-ras oncogene enhances karyotypic instability and integrates 
      predominantly in aberrant chromosomes.
PG  - 35-43
AB  - A human colon tumor cell line, SW480, was transfected with the c-Ha-ras oncogene, 
      the wild type c-Ha-ras gene, or the pSV2neo plasmid. Cytogenetic analysis and 
      localization of chromosome integration sites were combined in an attempt to 
      analyze the effects of transfection with the c-Ha-ras oncogene on the karyotype. 
      All transfected cell lines showed new clonal chromosome abnormalities present in 
      all cells, ranging from three new aberrations in pSV2neo-transfected SW480 cell 
      lines to eight in c-Ha-ras oncogene-transfected SW480 cell lines. The level of 
      expression of c-Ha-ras mRNA after transfection with the c-Ha-ras oncogene was 
      positively correlated with increased genetic instability, reflected in enhanced 
      karyotypic instability. A combination of banding and fluorescence in situ 
      hybridization (FISH) was used to identify chromosome integration sites. Plasmids 
      containing ras integrated predominantly in new structurally rearranged 
      chromosomes (five of eight). Three of five integration sites in new structurally 
      rearranged chromosomes were localized at or near translocation breakpoints 
      situated in telomeric regions. Specific chromosomes were not involved in the 
      chromosome rearrangements. The results indicate that 1) enhanced expression of 
      c-Ha-ras mRNA correlates with an increase in genetic instability in c-Ha-ras 
      oncogene-transfected SW480 cell lines, and 2) that no specific integration site 
      was observed but ras-containing plasmids were located predominantly in aberrant 
      chromosomes near or at translocation breakpoints involving telomeric bands.
FAU - de Vries, J E
AU  - de Vries JE
AD  - Department of Pathology, University of Limburg, Maastricht, The Netherlands.
FAU - Kornips, F H
AU  - Kornips FH
FAU - Marx, P
AU  - Marx P
FAU - Bosman, F T
AU  - Bosman FT
FAU - Geraedts, J P
AU  - Geraedts JP
FAU - ten Kate, J
AU  - ten Kate J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
GS  - c-Ha-ras
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Disorders
MH  - Chromosome Fragility
MH  - Colonic Neoplasms/genetics
MH  - Genes, ras/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Plasmids/genetics
MH  - RNA, Messenger/metabolism
MH  - RNA, Neoplasm/metabolism
MH  - *Transfection
MH  - Tumor Cells, Cultured
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 0165-4608(93)90041-J [pii]
AID - 10.1016/0165-4608(93)90041-j [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 May;67(1):35-43. doi: 10.1016/0165-4608(93)90041-j.