PMID- 8505063
OWN - NLM
STAT- MEDLINE
DCOM- 19930708
LR  - 20190830
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 38
IP  - 3
DP  - 1993
TI  - A cluster type organization of the loci of the immunoglobulin light chain in 
      Atlantic cod (Gadus morhua L.) and rainbow trout (Oncorhynchus mykiss Walbaum) 
      indicated by nucleotide sequences of cDNAs and hybridization analysis.
PG  - 199-209
AB  - Antibody screening and colony hybridization of cDNA libraries have been used to 
      isolate clones of the immunoglobulin light (IgL) chain from Atlantic cod (Gadus 
      morhua L.) and rainbow trout (Oncorhynchus mykiss Walbaum). Sequence analysis 
      shows dissimilarities in the constant part of the molecule (CL) within each 
      species. Comparisons of the amino acid sequences of the constant parts of the IgL 
      chains show a 55% identity between the two teleost species. When compared with 
      other species the highest similarities are found to the constant domain of the 
      IgL chain from mammals (30%-37%), but the teleost IgL chain can be classified 
      neither as kappa nor lambda. The VL domain in Atlantic cod and rainbow trout is 
      also more similar to those of mammals than to those of other animal species, but 
      no difference between kappa and lambda was noticed. Genomic Southern blots 
      hybridized with fragments coding for the constant part of IgL gave several bands 
      larger than 2 kilobases and a similar pattern was obtained with fragments coding 
      for the variable part. These results show that the locus of the IgL chain has a 
      multiple organization in teleost fish and that the locus has an organization 
      similar to that of sharks. Several of the cDNA clones isolated from both the head 
      kidney and the spleen represent nonrearranged or nonspliced mRNA, and northern 
      blot analysis shows that such transcripts are present in both the head kidney and 
      the spleen.
FAU - Daggfeldt, A
AU  - Daggfeldt A
AD  - Department of Immunology, Uppsala University.
FAU - Bengten, E
AU  - Bengten E
FAU - Pilstrom, L
AU  - Pilstrom L
LA  - eng
SI  - GENBANK/X65260
SI  - GENBANK/X68511
SI  - GENBANK/X68512
SI  - GENBANK/X68513
SI  - GENBANK/X68514
SI  - GENBANK/X68515
SI  - GENBANK/X68516
SI  - GENBANK/X68517
SI  - GENBANK/X68518
SI  - GENBANK/X68519
SI  - GENBANK/X68520
SI  - GENBANK/X68521
SI  - GENBANK/X68522
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Immunoglobulin Constant Regions)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - IgL
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA
MH  - Fishes/*genetics
MH  - Humans
MH  - Immunoglobulin Constant Regions/genetics
MH  - Immunoglobulin Light Chains/*genetics
MH  - Immunoglobulin Variable Region/genetics
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Nucleic Acid Hybridization
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Trout/genetics
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF00211520 [doi]
PST - ppublish
SO  - Immunogenetics. 1993;38(3):199-209. doi: 10.1007/BF00211520.