PMID- 8507874
OWN - NLM
STAT- MEDLINE
DCOM- 19930714
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 81
IP  - 12
DP  - 1993 Jun 15
TI  - Application of chromosome microdissection probes for elucidation of BCR-ABL 
      fusion and variant Philadelphia chromosome translocations in chronic myelogenous 
      leukemia.
PG  - 3365-71
AB  - Fluorescence in situ hybridization (FISH) has become an increasingly important 
      method for assessing chromosome rearrangement. The reciprocal translocation 
      constituting the Philadelphia (Ph) chromosome [t(9;22)(q34;q11)] characterizes 
      more than 90% of patients with chronic myelogenous leukemia (CML). However, in 
      the remaining cases the Ph chromosome (genetically characterized by the fusion of 
      the BCR-ABL genes) is thought to arise through complex translocations that are 
      often not readily apparent using routine chromosome-banding analysis. For this 
      reason we have developed unique band-specific probes for two-color FISH that 
      detect unequivocally the Ph chromosome, and its derivatives. Results of the 
      application of these probes are illustrated by analysis of 11 cases of CML (9 of 
      which contain "variant" translocations). The probes were generated by chromosome 
      microdissection and in vitro amplification of the bands involved in the Ph 
      translocation, leading to an extremely fast and sensitive approach to identify 
      this alteration in leukemic cell populations.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Radiation Oncology, University of Michigan Medical Center, Ann 
      Arbor 48109-0668.
FAU - Meltzer, P
AU  - Meltzer P
FAU - Jenkins, R
AU  - Jenkins R
FAU - Guan, X Y
AU  - Guan XY
FAU - Trent, J
AU  - Trent J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - *DNA Probes
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Molecular Sequence Data
MH  - *Philadelphia Chromosome
MH  - *Translocation, Genetic
EDAT- 1993/06/15 00:00
MHDA- 1993/06/15 00:01
CRDT- 1993/06/15 00:00
PHST- 1993/06/15 00:00 [pubmed]
PHST- 1993/06/15 00:01 [medline]
PHST- 1993/06/15 00:00 [entrez]
AID - S0006-4971(20)71907-5 [pii]
PST - ppublish
SO  - Blood. 1993 Jun 15;81(12):3365-71.