PMID- 8508674
OWN - NLM
STAT- MEDLINE
DCOM- 19930714
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 64
IP  - 1
DP  - 1993
TI  - Detection of aneuploidy in human interphase spermatozoa by fluorescence in situ 
      hybridization (FISH).
PG  - 23-6
AB  - Fluorescence in situ hybridization (FISH) with repetitive-sequence DNA probes was 
      performed on human interphase sperm to determine the utility of this technique 
      for aneuploidy detection. DNA sequences specific for chromosomes 15 and 16 and 
      the Y were biotinylated and hybridized with human sperm that had been treated 
      with cetyltrimethylammonium bromide and dithiothreitol to render them accessible 
      to the probes. Fluoresceinated avidin and antiavidin were utilized to visualize 
      bound probe. More than 10,000 sperm were analyzed for each chromosome probe. The 
      hybridization efficiency was 98-99% for all three probes. The frequencies for 
      disomy (i.e., for nuclei containing two fluorescent signals) for chromosomes 15 
      and 16 and the Y were 0.14%, 0.17%, and 0.11%, respectively. These frequencies 
      are all somewhat higher than those obtained by the human sperm karyotyping 
      technique. Nevertheless, these preliminary results suggest that FISH is a simple 
      and rapid technique that may provide an accurate screen for aneuploidy detection 
      in human sperm.
FAU - Martin, R H
AU  - Martin RH
AD  - Department of Pediatrics, Faculty of Medicine, University of Calgary, Alberta, 
      Canada.
FAU - Ko, E
AU  - Ko E
FAU - Chan, K
AU  - Chan K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 16
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Spermatozoa/ultrastructure
MH  - Y Chromosome
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1159/000133552 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1993;64(1):23-6. doi: 10.1159/000133552.