PMID- 8508679
OWN - NLM
STAT- MEDLINE
DCOM- 19930714
LR  - 20220129
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 64
IP  - 1
DP  - 1993
TI  - Fine mapping of the human MHC class II region within chromosome band 6p21 and 
      evaluation of probe ordering using interphase fluorescence in situ hybridization.
PG  - 49-53
AB  - Eight previously well-characterized and mapped probes derived from the human 
      major histocompatibility complex (MHC) class II region were used to investigate 
      the advantages and limitations of fluorescence in situ hybridization (FISH) 
      techniques for fine mapping. The class II region of the MHC was localized within 
      subband 6p21.31 by in situ hybridization on metaphase chromosomes that were 
      banded by immunofluorescence staining with an antibody against 
      5-bromodeoxyuridine (BrdU). Ordering of probes that were separated by up to 900 
      kb was achieved by simultaneous hybridization of two or three probes on 
      interphase nuclei. Three-color FISH proved to be an excellent method for ordering 
      of probes within distances of 200-1,000 kb. Under certain conditions, closer 
      probes could be ordered by comparing measured distances between their 
      hybridization signals in interphase nuclei. A linear correlation between measured 
      interphase distances and kilobase distances was observed up to 500 kb. With 
      increasing distances, the measurements become more inaccurate due to chromatin 
      folding.
FAU - Senger, G
AU  - Senger G
AD  - Human Cytogenetics Laboratory, Imperial Cancer Research Fund, London, UK.
FAU - Ragoussis, J
AU  - Ragoussis J
FAU - Trowsdale, J
AU  - Trowsdale J
FAU - Sheer, D
AU  - Sheer D
LA  - eng
GR  - A3585/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 6
MH  - Cosmids
MH  - DNA Probes
MH  - *Genes, MHC Class II
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphocytes/ultrastructure
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1159/000133559 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1993;64(1):49-53. doi: 10.1159/000133559.