PMID- 8511032
OWN - NLM
STAT- MEDLINE
DCOM- 19930715
LR  - 20071115
VI  - 35
IP  - 1
DP  - 1993 Feb
TI  - Improvement of cytogenetic results in B-cell chronic lymphocytic leukemia using 
      immuno-purified leukemic cells.
PG  - 103-5
AB  - Cytogenetics of purified B lymphocytes and whole blood cultures were studied in 
      14 patients with B-CLL and fluorescence in situ hybridization (FISH) was 
      performed on interphase cells in 12 of these cases in order to detect trisomy 12. 
      Only one minor abnormal clone was found on whole blood cell cultures, clonal 
      anomalies were found in a large proportion of mitoses of 6/14 cultures of 
      purified B cells. FISH detected a trisomy 12 in a case, not found in whole blood 
      cultures but found in 78% metaphases of cultures of purified B lymphocyte. This 
      method may be as sensitive as FISH for the detection of trisomy 12 and improves 
      the detection of clonal abnormalities in CLL.
FAU - Brizard, A
AU  - Brizard A
AD  - Laboratoire d'Hematologie, ER CNRS n.21, Centre Hospitalier Universitaire de 
      Poitiers, France.
FAU - Brizard, F
AU  - Brizard F
FAU - Lessard, M
AU  - Lessard M
FAU - Dreyfus, B
AU  - Dreyfus B
FAU - Guilhot, F
AU  - Guilhot F
FAU - Tanzer, J
AU  - Tanzer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Nouv Rev Fr Hematol (1978)
JT  - Nouvelle revue francaise d'hematologie
JID - 7909092
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Separation/*methods
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - Immunosorbent Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Trisomy
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PST - ppublish
SO  - Nouv Rev Fr Hematol (1978). 1993 Feb;35(1):103-5.