PMID- 8512560
OWN - NLM
STAT- MEDLINE
DCOM- 19930713
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 193
IP  - 2
DP  - 1993 Jun 15
TI  - Formation of compact globular particles in interphase nuclei from rat liver under 
      the effect of polyanions.
PG  - 591-8
AB  - Treatment of the isolated nuclei with polyanions (PA) (heparin and 
      dextran-sulfate) at the PA/DNA ratio of about 1 in the 0.15-0.5 M ammonium 
      acetate solution leads to rearrangement of the chromatin structure and formation 
      of compact globular particles (GP) 40-70 nm in diameter, bound by fibrils of 
      variable thickness (2-10 nm). GP formation is accompanied by a loss of 
      nucleosomal periodicity in DNA organization. However, no considerable loss of the 
      DNA contents has been found. At the PA/DNA ratio of more than 2, destruction of 
      the DNP-network and aggregation of GP in larger globular structures are observed. 
      The same effect is observed under the nuclease treatment. Under this condition 
      nuclei lose a considerable part of DNA, but retain histones supposedly as 
      histone-PA aggregates. Therefore the joint effect of PA and salt on nuclei at 
      certain conditions may lead to formation of artefact structures.
FAU - Prusov, A N
AU  - Prusov AN
AD  - A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 
      Russia.
FAU - Fais, D
AU  - Fais D
FAU - Polyakov VYu
AU  - Polyakov VYu
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chromatin)
RN  - 9005-49-6 (Heparin)
RN  - 9007-49-2 (DNA)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Cell Fractionation
MH  - Cell Nucleus/drug effects/*ultrastructure
MH  - Chromatin/drug effects/*ultrastructure
MH  - DNA/analysis
MH  - Dextran Sulfate/*pharmacology
MH  - Heparin/*pharmacology
MH  - Interphase
MH  - Liver/*cytology/drug effects/ultrastructure
MH  - Microscopy, Electron
MH  - Rats
EDAT- 1993/06/15 00:00
MHDA- 1993/06/15 00:01
CRDT- 1993/06/15 00:00
PHST- 1993/06/15 00:00 [pubmed]
PHST- 1993/06/15 00:01 [medline]
PHST- 1993/06/15 00:00 [entrez]
AID - S0006291X83716657 [pii]
AID - 10.1006/bbrc.1993.1665 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1993 Jun 15;193(2):591-8. doi: 
      10.1006/bbrc.1993.1665.