PMID- 8514385
OWN - NLM
STAT- MEDLINE
DCOM- 19930722
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 61
IP  - 7
DP  - 1993 Jul
TI  - Primary structure of and immunoglobulin E response to the repeat subunit of 
      gp15/400 from human lymphatic filarial parasites.
PG  - 2827-33
AB  - We have isolated and sequenced clones encoding the repeated subunit of the 
      surface-associated glycoprotein gp15/400 from the two nematode species 
      predominantly responsible for lymphatic filariasis in humans: Brugia malayi and 
      Wuchereria bancrofti. The amino acid sequence of the 15-kDa subunit, derived from 
      the nucleotide sequence of the gene fragment from B. malayi, is identical to that 
      previously reported for B. pahangi, whereas the derived W. bancrofti protein 
      sequence differs in only 7 of 132 residues. The identity of the protein in the 
      two Brugia species allowed us to use a recombinant from B. pahangi to examine the 
      serological response of adult Indonesian subjects infected with B. malayi. The 
      polymerase chain reaction-amplified subunit was expressed in Escherichia coli via 
      the pDS56/RBS11 plasmid and purified by nickel-chelating chromatography. A 
      significant proportion of individuals produced antigen-specific immunoglobulin E 
      (IgE). This was most pronounced in the individuals with elephantiasis, with 14 of 
      15 showing elevated titers and a mean of 3.2 ng of specific IgE ml-1. Only 2 of 
      15 microfilaremic individuals possessed elevated titers of specific IgE, with a 
      mean of 0.045 ng ml-1 for the group as a whole. Asymptomatic amicrofilaremic 
      residents showed approximately equal numbers of responders (defined as having a 
      value in the radioimmunoassay greater than two standard deviations above 
      controls) and nonresponders, with a group mean of 1.2 ng of antigen-specific IgE 
      ml-1.
FAU - Paxton, W A
AU  - Paxton WA
AD  - Department of Biochemistry, Wellcome Centre for Parasitic Infections, Imperial 
      College of Science, Technology and Medicine, London, United Kingdom.
FAU - Yazdanbakhsh, M
AU  - Yazdanbakhsh M
FAU - Kurniawan, A
AU  - Kurniawan A
FAU - Partono, F
AU  - Partono F
FAU - Maizels, R M
AU  - Maizels RM
FAU - Selkirk, M E
AU  - Selkirk ME
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (gp15-400 protein, Brugia pahangi)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Helminth/*biosynthesis
MH  - Antigens, Helminth/chemistry/immunology
MH  - Base Sequence
MH  - Brugia malayi/*immunology
MH  - Cloning, Molecular
MH  - Elephantiasis, Filarial/*immunology/parasitology
MH  - Helminth Proteins/chemistry/*immunology
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Male
MH  - Membrane Glycoproteins/chemistry/*immunology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Wuchereria bancrofti/immunology
PMC - PMC280927
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1993/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1128/iai.61.7.2827-2833.1993 [doi]
PST - ppublish
SO  - Infect Immun. 1993 Jul;61(7):2827-33. doi: 10.1128/iai.61.7.2827-2833.1993.