PMID- 8514386
OWN - NLM
STAT- MEDLINE
DCOM- 19930722
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 61
IP  - 7
DP  - 1993 Jul
TI  - Enhancement of interleukin-4 production by pertussis toxin.
PG  - 2834-40
AB  - Pertussis toxin (PT), a protein toxin of Bordetella pertussis, has many 
      biological activities, including potent adjuvant capacity and the ability to 
      up-regulate immunoglobulin E (IgE) production. Interleukin-4 (IL-4) is a cytokine 
      which is essential for the IgE response. Accordingly, we examined the effect of 
      PT on IL-4 production. Spleen and lymph node cell suspensions were prepared from 
      immunized mice and cultured with antigen or polyclonal stimuli in vitro. IL-4 
      secretion was assessed by bioassay, and IL-4 mRNA expression was assessed by 
      reverse transcription and polymerase chain reaction. Significantly larger amounts 
      of IL-4 protein and mRNA were produced in vitro by cells from mice given PT at 
      the time of immunization than by cells from mice given antigen alone, PT alone, 
      or the combination of antigen with cyclophosphamide. Total and antigen-specific 
      serum IgE levels were significantly elevated in immunized mice given PT, compared 
      with the other groups. Thus, there was a relationship between serum IgE and IL-4 
      production. The administration of a single dose of an anti-IL-4 monoclonal 
      antibody in vivo, at the time of immunization and treatment with PT, abolished 
      the development of the IgE response. These results indicate that PT is a potent 
      stimulus for the production of IL-4, which is required for the adjuvant effect of 
      PT on IgE formation.
FAU - Mu, H H
AU  - Mu HH
AD  - Centre for Immunology, St. Vincent's Hospital, Sydney, New South Wales, 
      Australia.
FAU - Sewell, W A
AU  - Sewell WA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Base Sequence
MH  - Female
MH  - Immunoglobulin E/biosynthesis
MH  - Interleukin-4/*biosynthesis/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Ovalbumin/immunology
MH  - *Pertussis Toxin
MH  - RNA, Messenger/analysis
MH  - Virulence Factors, Bordetella/*pharmacology
PMC - PMC280928
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1993/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1128/iai.61.7.2834-2840.1993 [doi]
PST - ppublish
SO  - Infect Immun. 1993 Jul;61(7):2834-40. doi: 10.1128/iai.61.7.2834-2840.1993.