PMID- 8516804
OWN - NLM
STAT- MEDLINE
DCOM- 19930720
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 55
IP  - 6
DP  - 1993 Jun
TI  - Selective intraportal hepatocyte transplantation in analbuminemic and Gunn rats.
PG  - 1213-9
AB  - Although significant progress has been achieved in isolated hepatocyte 
      transplantation, the optimal site of cell implantation has not yet been 
      determined. We have developed a novel experimental method of intraportal 
      hepatocyte transplantation that allows easy assessment of the morphology and 
      function of transplanted hepatocytes. Donor hepatocytes were harvested from 
      Sprague-Dawley rats by in situ EDTA/collagenase perfusion. Fifteen recipient 
      Nagase analbuminemic rats (NAR) underwent cannulation of the gastroduodenal vein 
      under ether anesthesia. Either the posterior or anterior liver lobes were 
      selectively infused with cells by occluding the portal venous supply of the 
      nontransplanted liver lobes. Normal donor hepatocytes (2 x 10(7)) suspended in 
      normal saline were infused over 1 min (4 ml). Recipients were treated with 
      cyclosporine for the duration of the experiment. Plasma albumin levels were 
      determined by ELISA, before and at various intervals after transplantation. In 
      NAR rats transplanted with normal hepatocytes, there was a significant (P < 
      0.003) and sustained (12 weeks) increase in plasma albumin levels. Control NAR 
      rats transplanted with NAR hepatocytes (n = 8) showed no significant changes in 
      plasma albumin levels. Similarly, normal Wistar hepatocytes were infused 
      intraportally into the posterior lobes of Gunn rats (n = 4), which lack the 
      ability to conjugate bilirubin. Pre- and posttransplantation bile was collected 
      following bile duct cannulation. Bile analysis by HPLC, demonstrated a 
      significant (P = 0.04) increase in the level of bilirubin conjugates following 
      transplantation and a corresponding decrease in total serum bilirubin (P = 0.04). 
      Our experimental data demonstrate that direct selective intraportal infusion of 
      hepatocytes is an effective technique of hepatocyte transplantation in the rat.
FAU - Holzman, M D
AU  - Holzman MD
AD  - Department of Surgery, Vanderbilt University School of Medicine, Nashville, 
      Tennessee.
FAU - Rozga, J
AU  - Rozga J
FAU - Neuzil, D F
AU  - Neuzil DF
FAU - Griffin, D
AU  - Griffin D
FAU - Moscioni, A D
AU  - Moscioni AD
FAU - Demetriou, A A
AU  - Demetriou AA
LA  - eng
GR  - DK38763-06/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Liver/cytology
MH  - Liver Transplantation/*methods/pathology
MH  - Male
MH  - Portal System
MH  - Rats
MH  - Rats, Gunn
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/deficiency
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.1097/00007890-199306000-00002 [doi]
PST - ppublish
SO  - Transplantation. 1993 Jun;55(6):1213-9. doi: 10.1097/00007890-199306000-00002.