PMID- 8518191
OWN - NLM
STAT- MEDLINE
DCOM- 19930729
LR  - 20190909
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 41
IP  - 2-3
DP  - 1993 May
TI  - Single-minded regulation of genes in the embryonic midline of the Drosophila 
      central nervous system.
PG  - 129-38
AB  - Proper development of the midline cells within the Drosophila central nervous 
      system is controlled by the single-minded (sim) gene. The sim protein defines a 
      new subclass of bHLH transcription factors and as such, is predicted to function 
      as a transcriptional regulator of other genes important for midline development. 
      Here we identify two potential targets of sim regulation: the sim gene itself, 
      and the 47F gene, which is expressed specifically in the sim-expressing midline 
      cells. Using a transgene containing sim regulatory sequences fused to 
      beta-galactosidase, we show that the sim gene uses two distinct promoters with 
      overlapping temporal specificities, and that expression from the late promoter is 
      autoregulated by sim itself. By expressing sim protein in salivary gland nuclei 
      using the heat-shock inducible Hsp70 gene promoter, we show that sim protein 
      associates with the sim late promoter on polytene chromosomes. In addition, sim 
      protein binds to a small number of additional chromosomal sites, among which are 
      the sites of the 47F gene, and two other genes, Tl and cdi, whose expression also 
      depend on sim function.
FAU - Muralidhar, M G
AU  - Muralidhar MG
AD  - Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San 
      Diego, CA 92186.
FAU - Callahan, C A
AU  - Callahan CA
FAU - Thomas, J B
AU  - Thomas JB
LA  - eng
GR  - GM07198/GM/NIGMS NIH HHS/United States
GR  - GM40613/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
GS  - 47F
GS  - Hsp70
GS  - Tl
GS  - cdi
GS  - f1p6
MH  - Animals
MH  - Binding Sites
MH  - Central Nervous System/*embryology
MH  - DNA-Binding Proteins/analysis/metabolism
MH  - Drosophila melanogaster/embryology/*genetics
MH  - Gene Expression Regulation
MH  - *Genes, Regulator
MH  - Heat-Shock Proteins
MH  - Morphogenesis/genetics
MH  - Promoter Regions, Genetic
MH  - Transcription Factors/metabolism
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 0925-4773(93)90043-W [pii]
AID - 10.1016/0925-4773(93)90043-w [doi]
PST - ppublish
SO  - Mech Dev. 1993 May;41(2-3):129-38. doi: 10.1016/0925-4773(93)90043-w.