PMID- 8518791
OWN - NLM
STAT- MEDLINE
DCOM- 19930727
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 2
IP  - 5
DP  - 1993 May
TI  - Isolation of cosmids and fetal brain cDNAs from the proximal long arm of human 
      chromosome 22.
PG  - 535-40
AB  - The proximal portion of human chromosome 22q appears to carry genes implicated in 
      the pathogenesis of various developmental disorders, including the cat eye 
      syndrome (CES) and the DiGeorge syndrome (DGS). A cosmid library was prepared 
      from a radiation hybrid selected for its content in chromosome 22 fragments. A 
      large fraction of cosmids containing human DNA were found to derive from the 
      juxtacentromeric region of chromosome 22, as shown by fluorescence in situ 
      hybridization (FISH) performed using individual cosmids or cosmid pools as 
      probes. Finer mapping was obtained for individual cosmids by hybridization to a 
      somatic cell hybrid mapping panel which splits the long arm of the chromosome 
      into 14 bins numbered 1 to 14 from the centromere to the telomere. Of the 10 
      cosmids mapped, eight belonged to group 1, the other two to group 14, in 
      agreement with FISH data. Rare endonuclease sites and fragments conserved between 
      species were searched in single cosmids, resulting in the selection of seven 
      cosmid fragments which were used to screen a human fetal brain cDNA library. 
      Three cDNAs were identified, encoded from two chromosome 22 genes which appeared 
      to be novel, as determined from partial end sequence and comparison with the 
      database entries. Fine localization of the 30.9 cDNA indicated that the 
      corresponding gene was located in a segment of proximal 22q overlapping with the 
      critical DGS region.
FAU - Lamour, V
AU  - Lamour V
AD  - Laboratoire de Biologie des Tumeurs Humaines, CNRS URA 1156, Institut Gustave 
      Roussy, Villejuif, France.
FAU - Levy, N
AU  - Levy N
FAU - Desmaze, C
AU  - Desmaze C
FAU - Baude, V
AU  - Baude V
FAU - Lecluse, Y
AU  - Lecluse Y
FAU - Delattre, O
AU  - Delattre O
FAU - Bernheim, A
AU  - Bernheim A
FAU - Thomas, G
AU  - Thomas G
FAU - Aurias, A
AU  - Aurias A
FAU - Lipinski, M
AU  - Lipinski M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - *Chromosomes, Human, Pair 22
MH  - Cosmids/genetics/*isolation & purification
MH  - Cricetinae
MH  - DNA/genetics/*isolation & purification
MH  - DiGeorge Syndrome/genetics
MH  - Fetus/metabolism
MH  - Gene Library
MH  - Genetic Vectors
MH  - Genome, Human
MH  - Humans
MH  - Hybrid Cells
MH  - Mice
EDAT- 1993/05/01 00:00
MHDA- 1993/05/01 00:01
CRDT- 1993/05/01 00:00
PHST- 1993/05/01 00:00 [pubmed]
PHST- 1993/05/01 00:01 [medline]
PHST- 1993/05/01 00:00 [entrez]
AID - 10.1093/hmg/2.5.535 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1993 May;2(5):535-40. doi: 10.1093/hmg/2.5.535.