PMID- 8522193 OWN - NLM STAT- MEDLINE DCOM- 19960122 LR - 20061115 IS - 0378-1119 (Print) IS - 0378-1119 (Linking) VI - 165 IP - 2 DP - 1995 Nov 20 TI - The human ribosomal protein S7-encoding gene: isolation, structure and localization in 2p25. PG - 297-302 AB - We have identified a gene encoding the human ribosomal protein (r-protein) S7. The S7 gene contains seven exons and six introns spanning about 6 kb. Organization of the gene is similar to that of Xenopus laevis S8, the only homologous intron-containing gene isolated so far. An mRNA transcribed from this gene has an open reading frame (ORF) of 582 nucleotides (nt), which encodes a protein of 194 amino acids (22.1 kDa). The transcription start point (tsp) was mapped by a primer extension assay to a C residue within a pyrimidine-rich tract. Human S7 (hS7) is identical to rat S7 (rS7) and exhibits significant similarity with the X. laevis, insect and plant homologs. We have used fluorescence in situ hybridization (FISH) to localize S7 to chromosome 2p25. FAU - Annilo, T AU - Annilo T AD - Institute of Molecular and Cell Biology, Tartu University, Estonian Biocentre, Estonia. FAU - Laan, M AU - Laan M FAU - Stahl, J AU - Stahl J FAU - Metspalu, A AU - Metspalu A LA - eng SI - GENBANK/L20096 SI - GENBANK/M77233 SI - GENBANK/Z25749 SI - GENBANK/Z47607 SI - GENBANK/Z47625 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Gene JT - Gene JID - 7706761 RN - 0 (RNA, Messenger) RN - 0 (Ribosomal Proteins) RN - 0 (ribosomal protein S7) RN - 0 (ribosomal protein S8) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Chromosome Mapping MH - *Chromosomes, Human, Pair 2 MH - Cloning, Molecular MH - Exons/genetics MH - Genes/*genetics MH - Humans MH - Introns/genetics MH - Molecular Sequence Data MH - Open Reading Frames/genetics MH - RNA, Messenger/biosynthesis MH - Rats MH - Ribosomal Proteins/*genetics MH - Sequence Alignment MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid MH - Transcription, Genetic/genetics MH - Xenopus laevis EDAT- 1995/11/20 00:00 MHDA- 1995/11/20 00:01 CRDT- 1995/11/20 00:00 PHST- 1995/11/20 00:00 [pubmed] PHST- 1995/11/20 00:01 [medline] PHST- 1995/11/20 00:00 [entrez] AID - 037811199500576R [pii] PST - ppublish SO - Gene. 1995 Nov 20;165(2):297-302.